双剂型双缩脲法测定血清总蛋白  

The Biuret Method for Total Protein of Serum Assay with Double Reagents

作　　者：吴教仁[1] 邱炼灼[1] 

机构地区：[1]潮州市中心医院,广东潮州521021

出　　处：《现代检验医学杂志》2004年第3期15-16,共2页Journal of Modern Laboratory Medicine

摘　　要：目的　为克服乳浊、黄疸、溶血对血清总蛋白测定的影响 ,将双缩脲法改为双剂型方法。方法　第一试剂为不含硫酸铜的双缩脲空白试剂 ,第二试剂系将原双缩脲试剂中的硫酸铜增加一倍 ,二者等量混合即成原法试剂。选取乳浊、黄疸、溶血及外观正常标本用新法与原法同时在日立 70 60自动生化分析仪上测定总蛋白含量。结果　血清总胆红素每增加 1 0 0 μmol/L,原法总蛋白增加 0 .7g/L,新法不受干扰 ;乳浊血清对原法结果影响十分明显 ,新法完全可排除乳浊干扰 ;标本溶血时 ,血红蛋白中的血红素与珠蛋白均对总蛋白测定结果造成明显影响 ,每 1 g/L血红蛋白可使原法总蛋白结果增加 2 .2 g/L,可使新法增加0 .8g/L;外观基本正常标本二法结果无显著差别。结论　双剂型双缩脲法在不改变原法反应原理 ,保留原法优点的基础上 ,能克服黄疸、乳浊的干扰 ,明显减少溶血的干扰 。Objective For conquering the interference of lipid turbidity,jaundice and hemolysis,changed simple reagent of biuret to double reagent (the method).Methods The first reagent was blank biuret reagent without cupric sulfate and cupric sulfate of second reagent was double than original reagent.When the two were mixed,it became the original reagent.Total protein of normal,lipid turbidity,jaundice and hemolysis specimens were assayed in Hitach 7060 with the method and original method.Results When total bilirubin in serum increased 100 u/L,total protein increased 0.7 g/L with original method,but it hadn't interference with this method;when the specimens was lipid turbidity,the interference of results were obvious with original method,but it has no interference with this method;when the specimens were hemolytic,the assay was influenced by the hemoprotein and hematohiston of hemoglobin.Every 1 g/L hemoglobin could make total protein increased 2.2 g/L with original method and 0.8 g/L with this method.The results had no obvious difference with two methods.Conclusion The principle of double reagent biuret is the same to original method,this reagent reserved original advantages.The interference of lipid turbidity and jaundice was conquered and the interference of hemolysis is obviously reduced.It is applicable to automatic analysis.

关 键 词：双试剂 双缩脲法 总蛋白 

分 类 号：R446[医药卫生—诊断学]