三种α-干扰素亚型结构与功能的研究  

Analysis on the structure and function of three IFN-α subtypes

作　　者：杜贤宇[1] 杨玲[1] 周业亭[1] 徐振山[1] 宋礼华[1] 

机构地区：[1]安徽省生物研究所,安徽合肥230031

出　　处：《疾病控制杂志》2004年第3期216-218,共3页Chinese Journal of Disease Control and Prevention

摘　　要：目的　利用自制的 6株单克隆抗体对α2a 干扰素、α2b 干扰素和α1b 干扰素的结构与功能进行分析。方法　采用细胞ELISA法、抗增殖活性和抗病毒活性等试验。结果　对干扰素抗病毒活性有影响的单克隆抗体对干扰素抗增殖活性也有影响 ,而对干扰素抗增殖活性有影响的 ,不一定对干扰素抗病毒活性有影响。结论　干扰素表面细胞受体结合位点与单克隆抗体结合位点是分开的。干扰素的抗病毒活性位点也是它的抗增殖活性位点 ,而干扰素的抗增殖活性作用的发挥可能还有其它位点的参与 ;单克隆抗体的中和活性不是通过阻断干扰素分子与细胞表面受体结合造成的。Objective To study structure and function of IFN-α2a, IFN-α2b and IFN-α1b. Methods 6 mAbs were used in antiproliferative activity, antiviral activity and ELISA assays. Results Results showed that mAbs affecting antiviral activity also affected antiproliferative activity while mAbs that affecting antiproliferative activity did not necessarily affect antiviral activity. ELISA results showed that the site binding cellular receptor was different from which binding mAbs. Conclusions Antiviral activity site of IFN was that of its antiproliferative activity, though some other sites might also be involved in the functioning of antiviral activity. The neutralizing activity of mAbs was not formed through preventing the binding of IFN molecules to cellular receptor.

关 键 词：Α-干扰素 亚型 α2a-干扰素 α2b-干扰素 Α1B-干扰素 抗病毒活性 蛋白质结构 

分 类 号：R978.7[医药卫生—药品]