阿齐霉素对生物膜铜绿假单胞菌的头孢他啶敏感性的作用(英文)  

Effect of azithromycin on the susceptibility of sessile Pseudomonas aeruginosa in biofilm to ceftazidime

作　　者：董荔[1] 何礼贤[1] 胡必杰[1] 王葆青[1] 陈雪华[1] 李华茵[1] 殷少军[1] 张海平[1] 

机构地区：[1]复旦大学附属中山医院呼吸病研究所,上海200032

出　　处：《中国抗生素杂志》2004年第6期366-370,共5页Chinese Journal of Antibiotics

摘　　要：铜绿假单胞菌 (PA)生物膜 (BF)是持续感染的重要原因之一。本研究采用结合 NCCL S标准的方法研究 BF中 PA的抗生素敏感性 ,主要研究了阿齐霉素对 BF中 PA抗生素敏感性的作用 ,为临床防治生物膜寻求潜在方法。本研究测定头孢服啶对悬浮状态和 BF中座生 PA(包括粘液型和非粘液型 )的最小杀菌浓度 (MBC)。在非粘液型菌株 ,悬浮菌和 BF菌均对头孢他啶敏感 ,加入 4μg/ ml阿齐霉素对头孢他啶的 MBC影响较小 ;在粘液型菌株 ,头孢他啶对 BF中的座生菌 MBC增高 ,而加入 4μg/ ml阿齐霉素明显降低了头孢他啶的 MBC,显示阿齐霉素增强了座生菌对头孢他啶的敏感性。Pseudomonas aeruginosa (PA) biofilm (BF) is one of important factor of persistent infections. A method combined with the NCCLS standards for testing antimicrobial susceptibility of PA in BF is studied. Azithromycin is tested for its effect on susceptibility of PA in BFs in order to search a potential method for clinical prevention of BF. The MBCs of ceftazidime on planktonic or sessile PA (mucoid and non-mucoid) are tested. The effects of 4μg/ml azithromycin on the MBCs of ceftazidime are studied. Ceftazidime shows similar MIC on mucoid or nonmucoid strains in routine microbroth dilution test. In nonmucoid strains,both planktonic and sessile bacteria are sensitive to ceftazidime and 4μg/ml azithromycin shows little effect on the MBCs of ceftazidime while in mucoid strains,sessile bacteria in biofilms show high MBCs of ceftazidime and adding 4μg/ml azithromycin brings obvious reduction in MBCs of ceftazidime and enhance the sessile bacterial sensitivity to ceftazidime.

关 键 词：铜绿假单胞菌 生物膜 阿齐霉素 头孢他啶 

分 类 号：R378.991[医药卫生—病原生物学] R978.1[医药卫生—基础医学]