用AFM对质粒DNA在云母表面的自组装研究  

Self-assembly behavior of plasmid DNA on the surface of mica studied with atomic force microscope

作　　者：王彬[1] 蔡继业[1] 孙彩军[2] 冯倩[1] 曾谷城[1] 梁志红[1] 潘善培[2] 

机构地区：[1]暨南大学化学系,广东广州510632 [2]暨南大学生殖免疫研究中心,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2004年第3期326-330,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家973项目(2001CB510101);国家自然科学基金(60278014);国家自然科学重点项目(30230350);广东省科技攻关项目(2002C32404)

摘　　要：通过原子力显微镜(AFM)对在云母表面上自然挥干的不同质量浓度的质粒DNA分子进行扫描,原子力显微镜图像显示:当质量浓度为0 1μg/mL时,DNA分子呈现出线性结构,随着质量浓度加大至1μg/mL,DNA分子相互缠绕为网状结构,质量浓度为10μg/mL时,呈现出具有典型的聚合形态,当质量浓度进一步加大到100μg/mL,则呈现出明显的树状脉络结构.通过原子力显微镜对质粒DNA分子的形态表征,可以看出质粒DNA分子具有典型的自组装的特性.Plasmid DNA at certain concentrations on mica were scanned by noncontact mode atomic force microscope. Linear topography was observed when plasmid DNA were at a concentration of 0.1 μg/mL. It was found to present a three-dimensional network on mica when at a concentration of 1 μg/mL and typical aggregate topography when at a concentration of 10 μg/mL. When the concentration reached 100 μg/mL, it showed tree-like structures. Typical self-assembly characteristic of plasmid DNA molecules on mica was observed by atomic force microscope.

关 键 词：原子力显微镜 脱氧核糖核苷酸 自组装 形态学 

分 类 号：Q523[生物学—生物化学]