叶酸快速检测方法及电化学分析综述  

A Review for Rapid Detection Methods and Electrochemical Analysis of Folic Acid

作　　者：李浩 林珍富 何文畅 陈柳君 陈悦欣 冯小珍[1] 

机构地区：[1]桂林电子科技大学生命与环境科学学院,广西 桂林

出　　处：《分析化学进展》2022年第2期157-164,共8页Advances in Analytical Chemistry

摘　　要：叶酸(Folic acid)是人体不可少的维生素,体液中叶酸浓度的降低会导致多种疾病。目前叶酸的检测方法种类繁多,包括微生物法、比色法、高效液相色谱法、荧光法、薄层层析法、离子捕获法、紫外分光光度法和电化学分析法等,本文对主要的快速检测方法进行了综述。其中较为主流的就是微生物法和高效液相色谱法,其简单易行且灵敏度高,非常适合低质量分数的叶酸检测。而在实验室的研究中,电化学分析由于可以在电脑上快速清楚地呈现电化学反应,非常便于分析其线性关系,同时它还具有高灵敏度、低成本和操作简便等优点,所以更加适用。另外,电化学方法还可以深层次研究叶酸的反应机理和动力学参数,因而有很大的发展潜力。Folic acid (FA) is an indispensable vitamin in human body. The reduction of FA concentration in body fluid will lead to a variety of diseases. At present, there are many detection methods of FA, including microbial method, high performance liquid chromatography (HPLC), colorimetry, fluorescence, thin layer chromatography, ion capture, ultraviolet spectrophotometry and electrochemical analysis. In this paper, we summarized the main rapid detection methods. Among them, microbial method and HPLC are the mainstream because they are simple, sensitive and very suitable for the detection of FA with low mass fraction. In laboratory research, however, electrochemical analysis is more suitable because it can quickly and clearly show the electrochemical reaction of FA on a computer, which is very convenient to analyze the linear relationship. Meanwhile, it also has the advantages of high sensitivity, low cost and simple operation. In addition, various methods of electrochemical analysis can also deeply study the reaction mechanism and kinetic parameters of FA, indicating great potential.

关 键 词：叶酸 检测方法 快速检测 电化学分析 

分 类 号：O657[理学—分析化学]