基于液相色谱分离保留因子计算的苯扎氯铵同系物辅助定性方法研究  

An Auxiliary Qualitative Analysis Method for Benzalammonium Chloride Homologous Compounds Based on Retention Factor Calculation

作　　者：沈宋利 卢珩俊 蔡振杭 鹿燕 叶涛 李珊珊 

机构地区：[1]浙江方圆检测集团股份有限公司化工产品检验所,浙江 杭州 [2]杭州市市场监督管理局,浙江 杭州

出　　处：《分析化学进展》2023年第2期121-126,共6页Advances in Analytical Chemistry

摘　　要：建立高效液相色谱分离,并用保留因子计算法定性分析苯扎氯铵同系物。采用高效液相色谱–二极管阵列检测器(HPLC-DAD)分离了4种烷基侧链长度为12~18 [碳链长度(C) = 12、14、16、18]的苯扎氯铵同系物。研究苯扎氯铵同系物侧链长度(C = 12, 14, 16, 18)与其调整保留时间(t,R)的相关关系。利用实验数据建立回归方程,并对样品中的未知苯扎氯铵样品的保留时间进行分析,同时改变流速、色谱柱、检测器和洗脱条件进行验证。结果表明,用该方法预测未知苯扎氯铵同系物的保留时间,误差范围 ≤ 1.25%。因此,可以利用保留因子定性分析未知苯扎氯铵同系物。In this paper, a qualitative analysis method for benzalammonium chloridehomologous compounds using HPLC separation and retention factor calculation was established. Four kinds of benzalammonium chloridehomologous compounds with aside chain length ranging from 12 to18 [side chain length (C) = 12, 14, 16, 18] were separated by high performance liquid chromatography with diode array detection (HPLC-DAD). Retention time ((t,R) of those unknown benzalammonium chloride samples was analyzed by the retention factor calculation based on the linear regression equation from experimental data. Different flow velocity, chromatographic column, detector and eluent conditions to verificate were also took into account. Results indicating that the error range are less than 1.25% in predicting the retention time of those unknown benzalammonium chloridehomologous compounds. Therefore, the retention factor is a useful method for qualitative analysis of unknown benzalammonium chloridehomologous compounds.

关 键 词：侧链长度 保留因子 苯扎氯铵 

分 类 号：R28[医药卫生—中药学]