补骨脂酊中活性成分补骨脂素、异补骨脂素薄层色谱鉴别  

Thinlayer Chromatography Identificatin of Active Components Psoralen and Iso-Psoralen in Psoralen Tincture

作　　者：王宇奇 陈亚林 

机构地区：[1]毕节市第一人民医院药剂科,贵州 毕节

出　　处：《分析化学进展》2023年第4期418-424,共7页Advances in Analytical Chemistry

摘　　要：目的:建立补骨脂酊中补骨脂素、异骨脂素鉴别方法。方法:采用薄层色谱法鉴别补骨脂酊中活性成分补骨脂素和异补骨脂素。结果:采用正乙烷:乙酸乙酯(3:1)、上行展开方式、点样量为1~2 μl,分离效果好,斑点清晰,操作简单快捷,重现性好。结论:通过分析影响薄层色谱鉴别因素,优化补骨脂素、异补骨脂素薄层色谱鉴别方法,为完善医疗机构制剂质量标准提供依据。Objective: To establish the identification method of psoralen and isopsoralen in psoralen tincture. Methods: Active components, namely, Psoralen and isopsoralen, were identified by thin-layer chromatography. Results: Using n-hexane:ethyl acetate (3:1) as the mobile phase, ascending de-velopment mode, and spot volume of 1~2 μl, good separation effect was achieved with clear and dis-tinct spots. The method was simple, fast, and exhibited good reproducibility. Conclusion: By analyz-ing the factors affecting thin-layer chromatography identification, the identification method for psoralen and isopsoralen was optimized, providing a basis for improving the quality standards of pharmaceutical preparations in medical institutions.

关 键 词：补骨脂酊 薄层色谱法 补骨脂素 异补骨脂素 

分 类 号：R28[医药卫生—中药学]