基于荧光法的DNA浓度测量方法研究  

Measurement of DNA Concentration Based on Fluorescence

作　　者：张振[1] 陈沁 刘张桃 曾媛 李振庆[1] 

机构地区：[1]上海理工大学光电信息与计算机工程学院,上海 [2]上海健康医学院分子影像学重点实验室,上海

出　　处：《分析化学进展》2024年第2期55-60,共6页Advances in Analytical Chemistry

摘　　要：DNA浓度测量在分子生物学中具有重要的应用价值。传统的基于吸光度法测量灵敏度低,需要试剂量大,为解决该问题,本文构建了基于荧光检测的高灵敏度微型DNA浓度测量系统。选取光源为中心波长505 nm激光模组,通过将SYBR Green I染料与DNA混合,研究了荧光强度与DNA浓度之间的量化关系。结果表明,DNA浓度在1~16 ng/μL范围内,与其荧光强度呈线性关系,相关系统高达0.994。本研究为小型DNA浓度测量系统的开发奠定了实验基础。DNA concentration measurement has important application value in molecular biology. The traditional absorbance-based measurement has low sensitivity and requires a large amount of reagents, in order to solve this problem, this paper constructed a highly sensitive micro DNA concentration measurement system based on fluorescence detection. The light source was selected as a laser module with a center wavelength of 505 nm, and the quantitative relationship between fluorescence intensity and DNA concentration was investigated by mixing SYBR Green I dye with DNA. The results showed that the DNA concentration was linearly related to its fluorescence intensity in the range of 1~16 ng/μL, and the correlation system was as high as 0.994. This study lays an experimental foundation for the development of a miniature DNA concentration measurement system.

关 键 词：DNA 荧光染料 浓度测量 

分 类 号：R28[医药卫生—中药学]