强腰舒络散的质量标准研究  

Study on the Quality Standard of Strong Waist and Relaxation

作　　者：柳长凤[1] 王倩楠 刘晓禹 

机构地区：[1]黑龙江中医药大学中医药研究院,黑龙江 哈尔滨 [2]黑龙江中医药大学研究生院,黑龙江 哈尔滨

出　　处：《临床医学进展》2023年第12期19822-19832,共11页Advances in Clinical Medicine

摘　　要：目的:建立强腰舒络散质量标准。方法:通过薄层色谱法,对制剂中的黄芪、川芎、当归、白芍、泽兰、醋莪术、防己和醋延胡索进行定性分析,以确定其有效成分。采用高效液相色谱法,对芍药苷、阿魏酸、毛蕊异黄酮葡萄糖苷和毛蕊花糖苷进行了精确的检测。结果:薄层色谱鉴别法斑点清晰,分离度高,阴性对照无干扰,表明其专属性好;含量测定显示,芍药苷在0.42356~1.48246 µg,阿魏酸在0.04188~ 0.14658 µg,毛蕊异黄酮葡萄糖苷在0.16288~0.57008 µg,毛蕊花糖苷在0.23020~0.80570 µg,线性关系良好(r ≥ 0.9990),仪器精密度、样品重复性、稳定性与加样回收率的RSD值均符合要求。结论:所创建的方法操作简单,重复性好,专属性强,可用于建立强腰舒络散的质量标准。Objective: To establish the quality standard of strong waist and relaxation. Methods: By thin layer chromatography, astrgali radix, chuanxiong rhizoma, Angelica sinensis radix, paeoniae radix alba, lycopi herba, Curcumae rhizoma, Stephania tetrandra radix and Corydalis rhizoma in the prepara-tion were qualitatively analyzed to determine their active ingredients. High performance liquid chromatography was used to accurately detect peonosides, ferulicacid, isoflavone glucosides and verbascoside by high performance liquid chromatography. Results: The thin layer chromatography identification method has clear spots, high resolution, and no interference from the negative con-trol, indicating that its specificity is good. The content determination showed that peony glycoside was 0.42356~1.48246 μg, ferulic acid was 0.04188~0.14658 μg, isoflavone glucoside was 0.16288~0.57008 μg, and verbascoside was 0.23020~0.80570 μg, with good linear relationship (r ≥ 0.9990), and the RSD values of instrument precision, sample repeatability, stability and dosing re-covery met the requirements. Conclusion: The created method is simple to operate, repeatable and exclusive, and can be used to establish quality standards for strong waist and relaxation.

关 键 词：强腰舒络散 质量标准 薄层色谱 高效液相 含量测定 

分 类 号：R28[医药卫生—中药学]