多功能水凝胶联合富血小板血浆对皮肤修复的研究  

Study on Multifunctional Hydrogel Combined with Platelet-Rich Plasma for Skin Repair

作　　者：李雅欣 刘红艳[1] 梁爽 王海燕[1] 

机构地区：[1]青岛大学附属医院输血科,山东 青岛

出　　处：《临床医学进展》2024年第3期1376-1382,共7页Advances in Clinical Medicine

摘　　要：目的:本实验拟制备一种具有优越的机械性、自愈性、粘附性、光热转化性和细胞相容性的智能纳米复合水凝胶,以增强伤口愈合过程。方法:首先通过柠檬酸钠还原法制备金纳米颗粒(AuNPs)溶液。接着,通过离心法获得富血小板血浆(PRP),将碳化钛(Ti3C2)和金纳米颗粒(AuNPs)引入到血小板水凝胶中,最终获得了金纳米/聚丙烯酰胺/碳化钛/富血小板血浆(AuNPs/PAM/Ti3C2/PRP)水凝胶。对水凝胶进行表征,证明其成功制备,并进一步研究其促进皮肤创面愈合的作用。结果:AuNPs/PAM/Ti3C2/PRP水凝胶呈疏松多孔状、孔径均一。且水凝胶具有良好的光热转化作用。体外实验证明AuNPs/PAM/Ti3C2/PRP水凝胶对细胞无明显毒害作用,而且还能促进细胞的增殖和迁移。结论:成功构建了具有拉伸性、自愈性、粘附性、光热转化性和细胞相容性的AuNPs/PAM/Ti3C2/PRP智能复合纳米水凝胶,其展现出良好的促细胞增殖和迁移的作用,在治疗皮肤损伤上具有很大的应用前景,有望成为新型伤口敷料。Objective: In this experiment, it is proposed to prepare a smart nanocomposite hydrogel with superior mechanical, self-healing, adhesion, photothermal conversion and cytocompatibility to enhance the wound healing process. Methods: Firstly, gold nanoparticles (AuNPs) solution was prepared by sodium citrate reduction method. Then, platelet-rich plasma (PRP) was obtained by centrifugation, and titanium carbide (Ti3C2) and gold nanoparticles (AuNPs) were introduced into the platelet hydrogel, and finally the gold nano/polyacrylamide/titanium carbide/platelet-rich plasma (AuNPs/PAM/Ti3C2/PRP) hydrogel was obtained. The hydrogels were characterized to demonstrate their successful preparation and further investigated for their role in promoting skin wound healing. Results: The AuNPs/PAM/Ti3C2/PRP hydrogels were loose and porous with uniform pore size. And the hydrogel has a good photothermal conversion effect. In vitro experiments demonstrated that AuNPs/PAM/Ti3C2/PRP hydrogels had no obvious toxic effects on cells and also promoted cell proliferation and migration. Conclusion: AuNPs/PAM/Ti3C2/PRP smart composite nanohydrogels with stretchability, self-healing, adhesion, photothermal conversion and cytocompatibility were successfully constructed, which exhibited good pro-cell proliferation and migration, and have great application prospects in the treatment of skin injuries, and are expected to become a new type of wound dressing.

关 键 词：金纳米颗粒 碳化钛 富血小板血浆 光热转化 皮肤损伤 

分 类 号：TB3[一般工业技术—材料科学与工程]