动脉粥样硬化中差异表达铁死亡相关基因的鉴定:生物信息学分析  

Identification of Differentially Expressed Ferroptosis-Related Genes in Atherosclerosis: Bioinformatics Analysis

作　　者：邹志毅 彭垒 王昆[1] 王士忠[1] 

机构地区：[1]青岛大学附属医院心血管外科,山东 青岛 [2]自贡市中医院外科,四川 自贡

出　　处：《临床医学进展》2024年第4期1514-1528,共15页Advances in Clinical Medicine

摘　　要：目的:铁死亡在动脉粥样硬化的发生和发展中起着至关重要的作用。本研究旨在已有研究的基础上,通过生物信息学分析进一步筛选鉴定与动脉粥样硬化中铁死亡相关的差异表达基因。材料方法:首先,mRNA表达谱数据集GSE100927从基因表达综合数据库中筛选,并使用R软件(4.0.0版)分析与动脉粥样硬化和铁死亡相关的潜在差异表达基因。随后,对选定的候选基因进行蛋白质–蛋白质相互作用分析、基因本体富集分析和京都基因与基因组百科全书通路富集分析等研究,最终构建受试者工作曲线,来预测其功能。结果:我们通过差异性分析确定了23个可能的与动脉粥样硬化铁死亡相关的潜在靶点。进一步通过基因本体富集分析发现这些候选基因的功能可能主要与氧化应激有关。蛋白质–蛋白质相互作用网络展示出绝大多数候选靶点存在密切的相互作用。最终我们再次从23个候选的潜在靶点中筛选了2个基因作为关键基因,即HMOX1和NOX4。结论:生物信息学分析鉴定了23个潜在的动脉粥样硬化铁死亡相关差异表达基因,而HMOX1和NOX4这2个基因可能作为关键基因在动脉粥样硬化过程中发挥更关键的作用。本研究结果可能有助于进一步了解动脉粥样硬化的发病机制,并为进一步指导临床治疗提供重要依据。Purpose: Ferroptosis plays a crucial role in the development and progression of atherosclerosis. The aim of this study was to identify differentially expressed genes associated with ferroptosis in atherosclerosis through bioinformatics analysis. Materials and Methods: First, the mRNA expression profile dataset GSE100927 was screened from the comprehensive gene expression database and analyzed using R software (version 4.0.0) for potential differentially expressed genes associated with atherosclerosis and iron death. Subsequently, protein-protein interaction analysis, gene ontology enrichment analysis and Kyoto Gene and Genome Encyclopedia pathway enrichment analysis were performed on the selected candidate genes, and finally the subject working curve was constructed to predict their functions. Results: We identified 23 possible potential targets associated with ferroptosis through differential analysis. The function of these candidate genes may be mainly related to oxidative stress through gene ontology enrichment analysis. Protein-protein interaction networks show close interactions at most candidate targets. Finally, we selected two key genes, namely HMOX1 and NOX4, from the 23 candidate potential targets. Conclusion: Bioinformatics analysis identified 23 potential differentially expressed genes associated with ferroptosis in atherosclerosis, two genes, HMOX1 and NOX4, may play a more critical role as key genes in the process of atherosclerosis. The results of this study may help to further understand the pathogenesis of atherosclerosis and provide an important basis for further clinical treatment.

关 键 词：动脉粥样硬化 铁死亡 NOX4 生物信息学分析 氧化应激 

分 类 号：R54[医药卫生—心血管疾病]