长春花细胞生物碱合成培养过程的营养消耗研究  

The Study of Nutrient Consumption on Catharanthus Roseus Cell in the Alkaloid Biosynthesis Culture

作　　者：赵楠 陈文[1] 郭志刚[1] 

机构地区：[1]清华大学化学工程系,北京

出　　处：《生物过程》2012年第2期58-64,共7页Bioprocess

基　　金：“十一五”国家科技支撑计划项目-植物细胞大规模培养及生物转化技术研究课题(2008BAI63B04).

摘　　要：本文利用EPC-500PE型植物细胞反应器培养了长春花细胞,筛选到较优的参数组合为150 r.p.m搅拌速度和0.5 mL/min供气量。在合成培养过程中,长春花细胞的蔗糖消耗量约为初始添加浓度(30 g/L)的50%,硝态氮的消耗量为初始添加浓度的90%,氨态氮的消耗量为初始添加浓度的50%,磷的消耗量为初始添加浓度的70%。长春碱含量在第5天达到峰值。Catharanthus roseus cells were cultured using the EPC-500 PE bio-fermenter, and a stirring speed of 150 r/min and gas supply of 0.5 ml/min were determined to be the best cultivating conditions. During the cultivation process, the cells’ sucrose consumption was about 50% of the initial addition (30 g/L), while nitrate consumption, ammonia nitrogen consumption and phosphorus consumption were 90%, 50% and 70% of the initial addition, respectively. The content of vinblastine peaked at the fifth day.

关 键 词：长春花 细胞培养 生物反应器 萜类吲哚生物碱 长春碱 

分 类 号：S5[农业科学—作物学]