大豆异黄酮转化菌株的分离及相关转化酶基因克隆研究进展  

Advances in Isolating of Isoflavone Biotransforming Bacteria and Cloning of Genes Encoding Relative Biotransforming Enzymes

作　　者：张红蕾[1] 于秀梅[1] 王秀伶[1] 

机构地区：[1]河北农业大学生命科学学院,保定

出　　处：《生物过程》2012年第2期98-104,共7页Bioprocess

基　　金：国家自然科学基金(No.31170058);河北省百名优秀创新人才支持计划(CPRC027)资助.

摘　　要：大量流行病学研究表明,大豆异黄酮具有明显的抗癌、抗氧化、抗炎、防护心脑血管以及预防骨质疏松等多种生理功能。被人和其他哺乳动物摄入体内的大豆异黄酮在胃肠道菌群作用下可被降解为二氢黄豆苷原、二氢染料木素、去氧甲基安哥拉紫檀素、雌马酚等不同代谢产物。体内及体外研究结果均表明,大豆异黄酮代谢产物具有比大豆异黄酮更高、更广的生物学活性。迄今,已有大量大豆异黄酮代谢产物产生菌从不同动物胃肠道微生物菌群中被分离出来。目前,人们正专注于从已分离的大豆异黄酮转化菌株中克隆相关转化酶基因。本文将对大豆异黄酮转化菌株分离及相关转化酶基因克隆现状作一综述。Epidemiological studies have shown that soy isoflavones have many important physiological activities, such as anticarcinogenic, antioxidant, antiinflammation, protection of cardiovascular diseases, prevention of osteoporosis etc. Isoflavones can be converted to different metabolites, including dihydrodaidzein (DHD), dihydrogenistein (DHG), O-desmethylangolensin (O-Dma), equol etc., by gastrointestinal microflora of humans and other mammals. Both in vitro and in vivo studies have indicated that isoflavone metabolites are of stronger and wider bioactivity than that of isoflavones themselves. To date, many specific bacterial strains capable of biotransforming isoflavones have been isolated from the microflora living in gastrointestinal tract of different animals. At present, researchers are focusing on cloning of genes encoding different biotransforming enzymes from isolated bacterial strains responsible for biotransforming isoflavones. This paper will review the isolated bacterial strains for biotransforming isoflavones and cloning of genes encoding for relative biotransforming enzymes.

关 键 词：大豆异黄酮 微生物转化 菌株分离 基因克隆 

分 类 号：R28[医药卫生—中药学]