高效液相色谱法测定海藻油中的DHA甲酯  

Determination of DHA-ME in Seaweed Oil by HPLC

作　　者：蔡凤珊 张琳 吴世凯[1,2] 

机构地区：[1]广州中国科学院先进技术研究所,广东省膜材料与膜分离重点实验室,广东广州 [2]中国科学院深圳先进技术研究院,广东深圳

出　　处：《生物过程》2016年第1期17-23,共7页Bioprocess

基　　金：深圳市技术创新计划技术开发项目[20140424111431];广东省产学研重点项目[2013B091300015]。

摘　　要：目的:建立高效液相色谱法分离测定海藻油样品中的二十二碳六烯酸甲酯(DHA甲酯)含量的方法。方法:采用Agilent Eclipse XDB-C18色谱柱(150 mm ×4.6 mm, 5 μm),以乙腈-水(92:8)溶液为流动相,流速为1.0 mL/min,检测波长205 nm。结果:DHA甲酯在10~500 μg/mL的范围内呈良好线性,线性回归方程为y = 2.586 ×103x + 5.927 ×103 (r2 = 1),平均回收率为94.23%,相对标准偏差为7.01%,海藻油中DHA-甲酯的平均含量为7.3876 mg/g。结论:本检测方法简便、快速、重现性好,可用于海藻油样品中DHA甲酯含量的测定。A method was developed for the determination of methyl-DHA in seaweed oil by high performance liquid chromatography (HPLC). The HPLC separation conditions were as follows: an Agilent Eclipse XDB-C18 column (150 mm ×4.6 mm, 5 μm) and elution with acetonitrile-water (v:v = 92:8), UV dection wavelength at 205 nm, 1.0 mL/min of flow rate for mobile phases. On the above conditions, good linearity was found over a concentration range of 10 - 500 μg/mL for methyl-DHA. Linear regression equation was y = 2.586 ×103x + 5.927 ×103 (r2 = 1). The average recovery rate was 94.23% (RSD = 7.01%). The average detection of methyl-DHA in seaweed oil was 7.3876 mg/g. This method was simple, rapid and high reproducibility;it can be used for the detection of methyl-DHA in seaweed oil.

关 键 词：DHA甲酯 海藻油 高效液相色谱 

分 类 号：R28[医药卫生—中药学]