纸质文物上一株交链孢霉生物学鉴定及其培养条件的优化  

The Biological Identification and Optimization of Culture Condition for a Alternaria Strain Mold on Paper Relics

作　　者：仲雨微 刘博 段大程[1] 刘辰澍 李其久[1] 贲松彬[1] 

机构地区：[1]辽宁大学生命科学院,辽宁沈阳 [2]辽宁省博物馆,辽宁沈阳 [3]辽宁省实验中学,辽宁沈阳

出　　处：《生物过程》2016年第1期24-34,共11页Bioprocess

基　　金：国家自然基金项目(31371085);辽宁省教育厅资助项目(2009A310);辽宁省科学技术计划项目(2010225036);辽宁大学国家自然基金预资助和博士启动项目共同资助。

摘　　要：目的:对带有“霉斑”的纸质文物上的霉菌进行分离鉴定并优化其培养条件。方法:通过形态学观察(三点植入法、镜下显微结构观察法)与分子生物学18S rDNA ITS序列扩增相结合对纸质文物上霉菌进行鉴定,采用单因素分析及正交试验对霉菌培养条件进行优化。结果:两种方法的鉴定结果均显示该菌株为链格孢霉菌(Alternaria eichhorniae),其培养最佳条件为28℃,pH 5.5,摇床震荡频率125 r/min。结论:确定该菌为链格孢霉菌(Alternaria eichhorniae),其培养条件实验室较易达到,为下一步纸制文物上的霉斑成份分析及生物法清洗霉斑的研究奠定基础,并为纸质文物上霉菌防治提供科学依据。Objective: To isolate and identify the fungus on paper heritage as well as optimized culture condi-tions. Method: Through morphology (three implantation, microscopic observation microstructure) and Molecular Biology 18S rDNA ITS sequence amplification a strain on the paper relics is identified. The fungal culture condition is optimized by using single factor analysis and orthogonal test. Results: Two identified results showed that it was a strain of Alternaria mold (Alternaria eichhorniae). The optimum for its cultivation conditions is 28?C, pH 5.5, shaking frequency 125 r/min. Conclusion: The strain is identified as a Alternaria mold (Alternaria eichhorniae) whose culture condition is easier to achieve in our laboratory, which makes basis for further analysis of components of mildew on paper artifacts and cleaning mildew with biological research, and also provides scientific basis for prevention and treatment of mold on paper artifacts.

关 键 词：霉菌 微生物 纸质文物 培养条件 优化 

分 类 号：S43[农业科学—农业昆虫与害虫防治]