低温冷冻保存对猪精子的影响  

Effect of Cryopreservation on Pig Sperm

作　　者：柴瑞兰 黄清松 徐晶 武艺 刘春霞[1,2] 李璐 王申元[1,2] 凌宇 张焱如[1,2] 周欢敏 韩雪峻[3] 曹俊伟 

机构地区：[1]内蒙古农业大学生命科学学院,内蒙古 呼和浩特 [2]内蒙古自治区生物制造重点实验室,内蒙古 呼和浩特 [3]上海祥欣畜禽有限公司,上海

出　　处：《生物过程》2021年第3期38-45,共8页Bioprocess

摘　　要：精子长期保存的最佳方法是低温冷冻保存,该方法具有保存时间长、生产成本低等优点。在养猪生产中广泛使用此项技术对精子进行长期保存,但由于猪精子质膜上的胆固醇/磷脂较低,所以较其他物种更容易受到低温的损伤,从而导致冷冻后的猪精子活力降低、受精率降低。本文就低温冷冻保存对猪精子超微结构、能量代谢等方面的影响进行了综述,阐明冻融精子的损伤机理,为提高冻精的活力奠定基础。The best method for long-term storageof semen is cryopreservation, which has the advantages of long storage time andlow production cost. This technology is widely used in pig production forlong-term preservation of sperm. But due to the low cholesterol/phospholipid onthe plasma membrane of pig sperm, therefore, compared with other species, theyare more vulnerable to low temperature damage, resulting in decreased sperm motilityand fertilization rate. In this paper, the effects of cryopreservation on theultrastructure and energy metabolism of porcine sperm were reviewed, and thedamage mechanism of frozen thawed sperm was elucidated, so as to lay afoundation for improving the vitality of frozen sperm.

关 键 词：猪精子 低温冷冻保存 

分 类 号：R69[医药卫生—泌尿科学]