直接半巢式PCR结合测序鉴定ApoE基因型的方法的建立  

Establishment of Method for Identifying ApoE Genotype by Direct Semi-Nested PCR Combined with Sequencing

作　　者：黄振辉 尹明星 游娟 何震宇 

机构地区：[1]广东药科大学药学院,广东 广州 [2]广东药科大学生命科学与生物制药学院,广东 广州

出　　处：《生物过程》2022年第4期199-204,共6页Bioprocess

摘　　要：目的:建立一种直接使用口腔上皮细胞为模板PCR扩增ApoE基因rs429358和rs7412位点从而采用桑格法测序鉴定ApoE基因型的方法。方法:自行设计3条引物,以口腔上皮细胞粗处理物为模板,通过半巢式PCR扩增包含ApoE基因2个SNP位点的靶片段,PCR产物经桑格法测序鉴定样本基因型。结果:所检样本能扩增出预期大小的PCR产物,测序峰图清晰。结论:成功建立了一种直接半巢式PCR结合测序鉴定ApoE基因型的方法,有良好的应用前景。Objective: To establish a method for identifying ApoE genotype by PCR and Sanger sequencing directly using oral epithelial cells. Meth-ods: Self-designed three primers were used to amplify the target fragments containing two SNP loci of ApoE gene by semi-nested PCR. The PCR products were sequenced to identify the genotype of the samples. Results: The PCR products of the expected size can be amplified from the tested samples, and the sequencing peak diagram is clear. Conclusion: A method of direct semi-nested PCR com-bined with sequencing to identify ApoE gene was successfully established, which has a certain ap-plication value.

关 键 词：APOE基因 直接半巢式PCR 测序 

分 类 号：Q78[生物学—分子生物学]