辐射诱变选育中华茉莉新品系  

Breeding of New Strains of Chinese Jasmine by Radiation Mutation

作　　者：宋勇 刘和民 邵云华[3] 宋涛[3] 战鑫 

机构地区：[1]山东省泰安徂徕山林场,山东 泰安 [2]山东省禾苗生物科技有限公司,山东 济南 [3]山东农业大学,山东 泰安

出　　处：《植物学研究》2021年第5期627-632,共6页Botanical Research

摘　　要：本研究用γ射线(60Co)辐射诱变处理“禾苗-1”中华茉莉胚性愈伤,茎、叶2种材料胚性愈伤诱导,茎的胚性愈伤诱导率高,达到100%。愈伤培养42 h胚性细胞分化,75~99 h胚性细胞大量形成,为γ射线最佳辐射处理时间,40 Gy辐射剂量较接近半致死剂量。变异体生根试管苗218株,经田间优选获得1个优系,暂定名为“禾苗-2”中华茉莉,连续2 y生长发育检测,性状稳定,明显高于亲本和对照。In this study, γ-ray (60Co) radiation mutagenesis was used to treat embryogenic callus of “He Miao-1” Chinese Jasmine. The embryogenic callus of stem and leaf were induced, and the induction rate of embryogenic callus of stem was high, reaching 100%. The callus was cultured for 42 hours of embryogenic cell differentiation, and a large number of embryogenic cells were formed at 75~99 hours. This is the best time for γ-ray radiation treatment, and the 40 Gy radiation dose is close to the half-lethal dose. 218 mutant rooting test-tube seedlings, one superior line was obtained by field optimization, tentatively named “He Miao-2” Chinese Jasmine, and the growth and development test for 2 years continuously showed stable traits, which were significantly higher than that of the parent and the control.

关 键 词：中华茉莉 胚性愈伤组织 变异体 Γ射线 半致死剂量 

分 类 号：S51[农业科学—作物学]