pH响应型克菌丹递送载体的制备及其对禾谷镰刀菌的抗菌活性  

Preparation of pH Responsive Carbendan Delivery Vector and Its Antibacterial Activity against Fusarium Graminearum

作　　者：王佳旭 王敬营 于秀敏[1] 王秀平[1] 

机构地区：[1]河北科技师范学院河北省作物逆境生物学重点实验室,河北 秦皇岛

出　　处：《植物学研究》2023年第3期130-141,共12页Botanical Research

摘　　要：传统农药制剂存在实际利用率低的问题,农药精准控释是农业可持续发展的关键。本论文制备了中空介孔二氧化硅(Hollow Mesoporous Silica Nanoparticles, HMSNs/H)装载克菌丹(Captan, Cap/C)并用壳聚糖(Chitosan, Chi)包覆作为克菌丹的递送体系,并研究了其对禾谷镰刀菌(Gibberella zeae (Schwein.) Petch)的杀菌活性。通过一锅法制备了装载克菌丹的中空介孔二氧化硅,并用壳聚糖对已经装载克菌丹的中空介孔二氧化硅进行包覆,使其具有pH响应功能。C/H@Chi粒径在250 nm左右,电位−18.64 mv。C/H@Chi在不同pH值(5.0, 6.0, 7.0)条件下,均表现出较好缓释性能。此外,C/H@Chi对禾谷镰刀菌表现出优异的抗菌活性,室内毒力测定试验表明Cap对禾谷镰刀菌菌丝生长抑制的EC50是2.21 mg/L,C/H@Chi对禾谷镰刀菌菌丝生长抑制的EC50是1.08 mg/L。安全性试验证明C/H@Chi对植物具有良好的生物安全性。There is a problem of low actual utilization rate of traditional pesticide preparations, and the precise and controlled release of pesticides is the key to the sustainable development of agriculture. In this paper, hollow mesoporous silica (Hollow Mesoporous Silica Nanoparticles, H) loaded with Captan (Captan, C) and coated with Chitosan (Chitosan, Chi) was prepared as the delivery system of captan, and its bioactivity against Fusarium graminearum (Gibberella zeae (Schwein.) Petch). the bactericidal activity of Petch. The hollow mesoporous silica loaded with captan was prepared by one-pot method, and the hollow mesoporous silica loaded with captan was coated with chitosan to make it have pH response function. The particle size of C/H@Chi is about 250 nm and the potential is −18.64 mv. C/H@Chi showed good sustained release performance at different pH values (5.0, 6.0, 7.0). In addition, C/H@Chi showed excellent antibacterial activity against Fusarium graminearum. Indoor toxicity test showed that the EC50 of Cap on mycelium growth of Fusarium graminearum was 2.21 mg/L, C/H@Chi, and the EC50 of Fusarium graminearum on mycelium growth was 1.08 mg/L. The safety test shows that C/H@Chi has good biosafety to plants.

关 键 词：中空介孔二氧化硅 克菌丹 壳聚糖 纳米农药 PH响应 

分 类 号：S43[农业科学—农业昆虫与害虫防治]