“绿岭”核桃种胚组培苗腋芽快繁体系的初步研究  

Rapid Propagation of “Lv Ling” Walnut (Juglans Regia L.) through Embryo-Induced Seedlings

作　　者：刘喜星 顾玉红[2] 李保国[1] 齐国辉[1] 刘璐[2] 回彦哲[1] 董丽欣[1] 马丽媛[1] 

机构地区：[1]河北农业大学林学院,保定 [2]河北农业大学生命科学学院,保定

出　　处：《农业科学》2012年第2期25-29,共5页Hans Journal of Agricultural Sciences

基　　金：国家林业公益性行业科研专项(201004093);河北省科技支撑项目(11230115D)。

摘　　要：以早实薄皮核桃“绿岭”成熟种胚诱导出的腋芽为外植体,MS为基本培养基,通过调节激素种类和浓度配比,初步研究了“绿岭”核桃种胚组培苗腋芽快繁体系。比较试验结果表明:最佳种胚萌发培养基为MS + 蔗糖30 g/L + 琼脂6 g/L;最佳腋芽诱导和增殖培养基为MS + 6-BA 2.0mg/L + NAA 0.2 mg/L + 蔗糖30 g/L + 琼脂6 g/L。生根采用两步生根法:先在DKW + IBA 8.0 mg/L + 蔗糖30 g/L + 琼脂6.0 g/L暗培养20 d,然后转入DKW + 蔗糖30 g/L + 琼脂6 g/L培养基中进行光照培养,生根率为50%。诱导生根的组培苗移栽到大田后成活率为33.3%。The mature embryo induced axillary buds were used as explants to investigate rapid propagation of precocious thin-shelled “Lv Ling” walnut in the MS basic medium with different concentrations of plant growth regulators. The results showed that MS + sucrose 30 g/L + agar6 g/L was the most feasible medium for embryo germination, and MS + 6-BA 2.0 mg/L + NAA 0.2 mg/L + sucrose30 g/L + agar6 g/L was the best for induction and multiplication of the axillary buds in all tested treatments. The 50% of rooting rate was achieved by a two-stage rooting method, namely, the shoots were first cultivated in DKW + IBA 8.0 mg/L + sucrose30 g/L + agar6.0 g/L medium for 20 days under dark condition, then transferred to DKW+ sucrose30 g/L + agar6.0 g/L medium under light condition. After transplanting to the field, 33.3% of the seedlings survived.

关 键 词：核桃 核桃属 胚 腋芽 激素 

分 类 号：S6[农业科学—园艺学]