利用花药和未受精子房培养技术创建二倍体光温敏雄性核不育系与恢复系  

Construction of Diploid Photoperiod-Sensitive Male Sterile Lines and Restorer Lines by Anthers and Unfertilized Ovary Culture Techniques

作　　者：童丽琦 王维 宋兆建[1] 张献华[1] 何玉池[1] 刘育华 蔡得田[1,2] 

机构地区：[1]湖北大学生命科学学院,湖北武汉 [2]武汉多倍体生物科技有限公司,湖北武汉

出　　处：《农业科学》2017年第4期281-287,共7页Hans Journal of Agricultural Sciences

基　　金：国家自然科学基金资助项目(31271690, 31571639);湖北省自然科学基金资助项目(2015CFB295);武汉市品牌农业发展计划项目。

摘　　要：[目的] 利用远缘杂交和多倍体双重优势选育水稻新品种是育种的新途径。在选育出多倍体减数分裂稳定性品系,解决多倍体结实率低的瓶颈问题后,开展多倍体水稻的理论和育种实践迫切需要合适的育种材料。[方法] 本文以多倍体水稻光温敏雄性核不育系PS001和多倍体水稻恢复系PR-T1为实验材料,分别对其进行花药和未受精子房培养而得到二倍体。[结果] 从而构建成二倍体和四倍体配套的光温敏水稻雄性核不育系和恢复系材料。[结论] 证明了利用花药和未受精子房培养既可以快速纯化品种,缩短性状稳定年限,达到快速育种的目的,又可以创造合适的用于理论研究的材料。[Objective] Using double heterosis of polyploidization and distant hybridization to select new rice varieties is a new breeding strategy. After breeding the polyploid meiosis stability line and solving the bottleneck problem of low seed setting rate of polyploidy, the theory and breeding practice of polyploid rice urgently need suitable breeding materials. [Method] In this paper, the photoperiod and thermo-sensitive genic male sterility of PS001 and polyploid rice restorer line PR-T1 are used as experimental materials. They are used to anther and unfertilized ovary culture, and to get their diploid, respectively. [Result] In this paper, it creates a set of lines that contains diploid and tetraploid. The lines are photoperiod and thermo-sensitive genic male sterility and restorer line. [Conclusion] It is concluded that the anther and unfertilized ovary culture could be used to rapidly purify varieties and shorten the stable time of traits to achieve the purpose of rapid breeding, but also to create the appropriate materials for theoretical research.

关 键 词：多倍体水稻 花药和未受精子房培养 光温敏雄性核不育系 愈伤组织 二倍体品系 

分 类 号：S5[农业科学—作物学]