一种土壤/沉积物中2-甲基苯并噻唑的分析方法  

An Analytical Method of 2-Methylbenzothiazole in Soil/Sediment

作　　者：张席席 王继忠 黄贵凤 邹敬 

机构地区：[1]广电计量检测(合肥)有限公司,安徽 合肥 [2]安徽建筑大学,环境与能源工程学院,安徽 合肥

出　　处：《农业科学》2021年第11期1073-1078,共6页Hans Journal of Agricultural Sciences

摘　　要：建立了一种土壤中2-甲基苯并噻唑的前处理和定量方法,其主要原理如下:土壤样品经自然风干后,称取10.0 g样品放在玻璃纤维滤膜内,包裹起来,放入索氏提取器中,用100 mL二氯甲烷:丙酮(1:1)索氏抽提18 h,收集提取液,并通过无水硫酸钠除去提取液中的水分,然后用旋转蒸发仪中浓缩至1 mL后,加入过量正己烷(10 mL),交换溶剂,并再次浓缩至1.0 mL,浓缩液通过内径为1.0 cm填充3.0 g硅胶(100~200目)的层析柱,并加入30 mL正己烷:二氯甲烷(1:1)淋洗,收集淋洗液,浓缩定容至1.0 mL,通过气相色谱–质谱外标法定量。A pretreatment and quantitative method for 2-methylbenzothiazole in soil was established. The main principle is as follows: after natural air drying, place 10.0 g of soil sample in glass fiber filter membrane, wrap and put it into Soxhlet extractor. Soxhlet extraction with 100 mL dichloro-methane:acetone (1:1) for 18 hours, collect the extract, and remove the water in the extract through anhydrous sodium sulfate. Then concentrate to 1 mL with a rotary evaporator, add excess n-hexane (10 mL), exchange the solvent, and concentrate again to 1.0 mL. After the concentrated solution passes through a chromatographic column filled with 3.0 g silica gel (100~200 mesh) with an inner diameter of 1.0 cm, add 30 mL n-hexane:dichloromethane (1:1) for leaching. Collect the leaching solution, concentrate to volume to 1.0 mL, and quantify it by gas chromatography-mass spectrometry external standard method.

关 键 词：2-甲基苯并噻唑 土壤/沉积物 前处理 气相色谱–质谱 

分 类 号：R28[医药卫生—中药学]