基因芯片和单一RT-PCR在甘薯试管苗病毒检测中的应用  

Application Analysis of Gene Chip and Single RT-PCR in Virus Detection of Sweet Potato Tube Plantlet

作　　者：侯夫云[1] 翟会青 王庆美[1] 秦桢[1] 周媛媛 李爱贤[1] 

机构地区：[1]山东省农业科学院作物研究所/农村农业部黄淮海薯类科学观测实验站(济南),山东 济南 [2]石家庄慧谷农业科技有限公司,河北 石家庄

出　　处：《农业科学》2022年第8期704-708,共5页Hans Journal of Agricultural Sciences

摘　　要：以120份不同甘薯品种试管苗为材料,利用单一RT-PCR和基因芯片两种技术进行病毒检测比较研究。单一RT-PCR结果表明,供试样品中甘薯曲叶病毒SPLCV的阳性率最高,达62.5%;依次是甘薯褪绿矮化病毒SPCSV、甘薯羽状斑驳病毒SPFMV、甘薯G病毒SPVG以及潜隐病毒SPLV。基因芯片在SPLCV、SPCSV、SPFMV及SPVG等4种病毒的检出率结果显著低于单一RT-PCR。因此,单一RT-PCR较适用于试管苗病毒检测,基因芯片快速、便捷、高通量,但是对甘薯病毒的检测尚需进一步优化。120 tube plantlets of sweet potato were used as materials to detect sweet potato virus by single RT-PCR and gene chip. Single RT-PCR results showed that the positive rate of sweet potato curl virus (SPLCV) was the highest up to 62.5%. The detection rates of sweet potato chlorotic stunt virus (SPCSV), sweet potato feathery mottle virus (SPFMV), sweet potato virus G (SPVG) and Sweet potato latent virus (SPLV) decreased successively. The detection rates of SPLCV, SPCSV, SPFMV and SPVG using gene chips were significantly lower than single RT-PCR. Single RT-PCR has strong specificity, good repeatability and good stability. However, single RT-PCR is more suitable for detecting sweet potato virus, gene chip has the characters of fast, convenient and high throughput, but its applica-tion in sweet potato is not mature yet and the detection of SPLCV, SPCSV and SPFMV needs further optimization.

关 键 词：甘薯 单一RT-PCR 基因芯片 病毒 

分 类 号：S531[农业科学—作物学]