蚕丝纬编针织网的细胞相容性及体外降解性实验研究  

Experimental Study on Cytocompatibility and in Vitro Degradability of Silk Weft Knitted Mesh

作　　者：唐靓 李跃中 张文元 

机构地区：[1]杭州医学院检验医学院、生物工程学院,浙江 杭州 [2]杭州医学院药学院、食品科学与工程学院,浙江 杭州

出　　处：《生物医学》2024年第4期555-562,共8页Hans Journal of Biomedicine

摘　　要：目的:蚕丝纬编针织网支架结合骨髓间充质干细胞(BMSCs)细胞片制成组织工程支架,并对其进行细胞相容性观察,以及蚕丝纬编针织网支架的体外降解性。方法:通过密度梯度离心法结合贴壁培养法提取兔BMSCs,通过维生素C培养法制备BMSCs细胞片。采用纬编针织法制备蚕丝网状支架,将BMSCs细胞片覆盖于该支架上体外培养2周,观察细胞在支架上的生长情况。并进行蚕丝纬编针织网支架为期1年的体外降解性的力学性能及质量变化测试。结果:提取的BMSCs生长旺盛。支架-BMSCs细胞片培养2周的扫描电镜观察显示:BMSCs黏附于支架呈立体生长,增殖良好。通过体外1年降解实验,蚕丝纬编针织网状支架降解速率非常缓慢,力学性能及质量变化很小。结论:蚕丝纬编针织网-BMSCs细胞片具有良好的细胞相容性,蚕丝纬编针织网体外降解速率非常缓慢,可以尝试作为组织工程韧带/肌腱的支架材料。Objective: Silk weft knitted mesh scaffolds were combined with bone marrow-derived mesenchymal stem cells (BMSCs) to form tissue engineering scaffolds, and the cytocompatibility and degradability of silk weft knitted mesh scaffolds were observed. Methods: BMSCs were extracted by density gradient centrifugation combined with adherent culture, and BMSCs cell sheets were prepared by vitamin C culture. A silk mesh scaffold was prepared by weft knitting method. BMSCs cell sheets were covered on the scaffold and cultured for 2 weeks in vitro to observe the growth of cells on the scaffold. The mechanical properties and quality changes of silk weft knitted mesh scaffolds were tested for 1 year. Results: The extracted BMSCs grew strongly. Scanning electron microscopy (SEM) observation of BMSCs cells cultured on scaffolds for 2 weeks showed that BMSCs adhered to scaffolds in stereoscopic growth and proliferation. After 1 year in vitro degradation experiment, the degradation rate of silk weft knitted mesh scaffold is very slow, an

关 键 词：蚕丝纬编针织网 骨髓间充质干细胞 细胞片 细胞相容性 体外降解性 力学性能 

分 类 号：R32[医药卫生—人体解剖和组织胚胎学]