基于均匀设计法优化骆驼蓬抗氧化性物质提取工艺  

Optimization of the Extraction Technology of Antioxidant Substances from the Peganum harmala L. by Uniform Design

作　　者：李茵萍[1] 张艳慧[1] 古丽美克热依·吐尼亚孜 德丽达·木拉提别克 迪丽努尔·马力克[1] 何清 耿珠峰 

机构地区：[1]新疆师范大学,化学化工学院,新疆乌鲁木齐 [2]天津大学,化工技术学院,天津 [3]北京师范大学,分析测试中心,北京

出　　处：《计算生物学》2019年第3期52-58,共7页Hans Journal of Computational Biology

基　　金：新疆师范大学博士科研启动基金(No.XJNUBS1901);新疆师范大学自治区教学改革与研究项目(No.ZJG2019-06).

摘　　要：为探寻骆驼蓬中抗氧化性物质的最佳提取方法,采用不同极性溶剂提取与均匀设计法相结合进行试验。以骆驼蓬为研究对象,DPPH清除自由基抗氧化活性抑制率为测试指标,对均匀设计实验数据进行二次多项式逐步回归分析。结果筛选甲醇作为最佳提取溶剂,其抑制率为76.64%。得到最优提取工艺条件:提取3次,提取时间20 min,料液比1:14 g/mL,通过验证此时骆驼蓬的甲醇提取物DPPH清除自由基抗氧化活性的抑制率为75.13%,为最佳提取工艺。In order to optimize the extractionprocess of antioxidants in Peganumharmala L.,different solvent extraction combined uniform design methodwere employed in this research.With the inhibition rate of DPPH scavengingfree radical antioxidant activity as the test index,the experimental data ofuniform design were analyzed by quadratic polynomial stepwise regression.Methanol was selected to be the optimum extraction solvent with an inhibitionof 76.64%.The optimum extraction conditions are as follows:extract is 3 times;extraction time is 20 min;liquid-solid ratio is 1:14 g/mL.At this time,the inhibition rate of scavenging DPPH freeradical antioxidant activity of methanol extract from Peganum harmala L.was 75.13%,which was the best extraction process.

关 键 词：骆驼蓬 提取溶剂 均匀设计法 DPPH抗氧化活性 

分 类 号：R28[医药卫生—中药学]