灵菌红素的纯化制备工艺研究  

The Study on the Preparation and Purification of Prodigiosin

作　　者：葛金祥 孙诗清[1] 冯德明[1] 林德镇 吴轶 

机构地区：[1]嘉兴学院南湖学院,浙江嘉兴

出　　处：《化学工程与技术》2019年第3期282-287,共6页Hans Journal of Chemical Engineering and Technology

基　　金：嘉兴市科技局项目(2016AY13005)资助;2018年嘉兴学院重点SRT项目(85178404)资助。

摘　　要：通过微生物发酵法生产灵菌红素,薄层层析法筛选合适的灵菌红素展开剂,然后利用硅胶柱层析高效纯化制备灵菌红素纯品,高效液相色谱法检测各纯化组分中灵菌红素的含量。结果发现,微生物发酵法生产灵菌红素粗产品达到6.23 ±1.56 g/L,其含量为0.29% ±0.14%;丙酮提取物达到52.3 ±4.5 mg/L,其含量为29.6% ±4.8%;以氯仿与乙酸乙酯为基础筛选出9:1比例的灵菌红素与杂质的分离效果较好,适合作为硅胶柱层析的洗脱剂进行灵菌红素的纯化,纯化后的产品经过乙醚洗涤结晶后,产量达到3.3 ±0.4 mg/L,其含量为96.2 ±1.3%。In this work,prodigiosin from microbial was purified by silica gel column chromatography,and suitable eluents for prodigiosin were screened by TLC.The content of prodigiosin in different pu-rified steps was determined by high performance liquid chromatography.The results showed that the crude product of prodigiosin produced by microbial fermentation reached 6.23±1.56 g/L with the content of 0.29%±0.14%;the acetone extract reached 52.3±4.5 mg/L with the content of 29.6%±4.8%;the ratio of suitable eluent was 9:1(chloroform and ethyl acetate).The final content of prodigiosin could reach 96.2%±1.3%and its yield was 3.3±0.4 mg/L.

关 键 词：灵菌红素 分离纯化 高效液相 

分 类 号：R28[医药卫生—中药学]