烷化剂甲磺酸甲酯(MMS)对SD大鼠心血管系统的影响  

Effects of the Alkylating Agent Methyl Methanesulfonate on the Cardiovascular System in SD Rats

作　　者：蒋天靓 干铁儿[2] 徐贤荣[1] 曹亦菲[1] 李红娟[1] 杨军[1,3] 

机构地区：[1]杭州师范大学医学院预防医学系,浙江杭州 [2]浙江省中医院院感科,浙江杭州 [3]浙江大学医学院附属妇产科医院子宫癌诊疗中心,浙江杭州

出　　处：《生理学研究》2019年第3期17-24,共8页Journal of Physiology Studies

基　　金：国家自然科学基金青年基金(81602795);浙江省自然科学基金(LZ19H260001,LQ15H26002,LY19H260002)。

摘　　要：目的:探讨DNA损伤剂甲磺酸甲酯(MMS)对SD大鼠心血管系统的影响。方法:采用腹腔注射方式给予SD大鼠甲基磺酸甲酯(25 mg/kg或60mg/kg)。经1天或3天后处死大鼠,并检测以下指标:外周血细胞数,血压,心率,血管壁超微结构,胸主动脉张力以及血小板聚集情况。结果:研究显示,与对照组相比,在两个剂量组中MMS均能够显著减少外周血白细胞数量;但是心率和血压则不受影响。此外,MMS对胸主动脉张力没有影响,电镜分析也发现其对胸主动脉血管壁超微结构没有损伤。最后,对对照组和MMS处理组进行ADP诱导的血小板聚集反应分析发现,两组之间差异无统计学显著性。结论:综上所述,不同于MMS的遗传毒性特征,其对SD大鼠的心血管系统无明显毒性。Objective:To investigate the effectsof DNA damage agent methyl mesylate(MMS)on cardiovascular system inSprague-Dawley(SD)rats.Methods:SD rats were intraperitoneally injected withMMS(25 or 60 mg/kg).One day or 3 days later,rats were sacrificed and the followingparameters were measured and examined:peripheral blood cell count,bloodpressure,heart rate,ultrastructure of the vascular wall,thoracic aortatension,and platelet aggregation.Results:It was found that MMS couldsignificantly decrease the white blood cell counts at both doses compared to controls.On the other hand,heart rate and blood pressure were not affected.In addition,MMS had no effect on the tension in thoracic aorti,and electromicroscopyanalysis also revealed no significant damage to the thoracic aorticultrastructure.Finally,ADP-induced platelet aggregation was evaluated in bothMMS-treated and control rats,and the results showed no significant differencebetween the two groups.Conclusion:Taken together,it is suggested that MMSdoes not have any significant effect on the cardiovascular system in the ratmodel.

关 键 词：甲磺酸甲酯 心血管毒性 血管张力 血小板聚集 

分 类 号：R5[医药卫生—内科学]