育阴散对达那唑诱导的雌性性早熟大鼠的治疗作用的研究  

Study on the Therapeutic Effect of Yuyinsan on Female Precocious Puberty Rats Induced by Danazol

作　　者：吴博[1] 陈冬梅[1] 郑怜玉 陈传强 杨文庆[1] 

机构地区：[1]福建中医药大学附属第二人民医院儿科,福建 福州

出　　处：《亚洲儿科病例研究》2024年第2期7-11,共5页Asian Case Reports in Pediatrics

摘　　要：目的 观察育阴散干预达那唑诱导的雌性性早熟大鼠模型的影响。方法 将雌性幼龄SD大鼠40只,按照随机数字表法分为正常组、模型组、生理盐水干预组和中药干预组,每组10只。除正常组外,其余三组大鼠均在5日龄采用皮下注射达那唑300 μg的诱导方法建立性早熟模型,生理盐水干预组和中药干预组在15日龄时用生理盐水和育阴散进行干预,同时观察记录各组大鼠阴门开启情况。待模型组大鼠阴门开启后,对各组大鼠进行子宫和卵巢指数进行比较,并用酶联免疫吸附法检测各组大鼠血清中LH、E2的水平,分析育阴散干预雌性性早熟模型大鼠对其性器官和性激素的影响。结果 与正常组和中药干预组比较,模型组和生理盐水干预组大鼠阴门开启时间提前。与正常组比较,模型组和生理盐水干预组大鼠的子宫和卵巢指数明显升高(P 0.05),但血清E2水平则升高(P Objective To observe the effect of Yuyinsan on the female precocious puberty rat model induced by danazol. Methods Forty young female SD rats were divided into a normal group, a model group, a saline intervention group, and a Chinese medicine intervention group according to the random number table method, with 10 rats in each group. Except for the normal group, the other three groups of rats were all treated with subcutaneous injection of 300 μg of danazol to establish the precocious puberty model at 5 days of age. The saline intervention group and the Chinese medicine intervention group were intervened with saline and Yuyinsan at 15 days of age, and the opening of the vulva of the rats in each group was observed and recorded. After the vulva of the model group rats opened, the uterine and ovarian indexes of the rats in each group were compared, and the levels of LH and E2 in the serum of the rats in each group were detected by enzyme-linked immunosorbent assay to analyze the effects of Yuyinsan on the sexual organs and sex hormones of female precocio

关 键 词：性早熟 达那唑 大鼠 育阴散 中药 

分 类 号：R28[医药卫生—中药学]