基于肌红蛋白/海藻酸钙微球修饰的过氧化氢生物传感器  

A Biosensor for Hydrogen Peroxide Based on Myoglobin Immobilized on Calcium Alginate Microspheres

作　　者：胡娜 孙红 Na Hu;Hong Sun(College of Chemistry and Chemical Engineering, Qingdao University, Qingdao Shandong;College of Materials Science and Engineering, Qingdao University, Qingdao Shandong)

机构地区：[1]青岛大学化学化工学院,山东青岛 [2]青岛大学材料科学与工程学院,山东青岛

出　　处：《材料科学》2019年第2期170-176,共7页Material Sciences

摘　　要：在该研究中,通过使用海藻酸钠,氯化钙,聚乙二醇和聚乙烯醇,制备了新型的海藻酸钙微球(CA MS),并通过在电极表面上与肌红蛋白(Mb)组装构建了Mb/CA微球过氧化氢(H2O2)生物传感器。用循环伏安法和计时电流法检测了该生物传感器的电化学性能。在磷酸盐缓冲溶液(pH = 7)中,Mb/CA微球膜修饰电极在约?0.358 (vs饱和甘汞电极(SCE))显示出一对明显且对称性良好的氧化还原峰。此外,该生物传感器在30天后仍可保持良好的活性和灵敏度。实验结果表明,该生物传感器对H2O2的还原表现出了良好的电催化性能,在信噪比(S/N)等于3的情况下该生物传感器的线性扫描范围为6.25 μM到50 μM,线性相关系数R2 = 0.996,检测限为1.25 μM,并具有良好的稳定性、重现性、选择性等优点。因此,该构建的生物传感器可以用于实际环境中污染物的精确测定。In this research, by using sodium alginate, calcium chloride, polyethylene glycol and polyvinyl al-cohol, a novel calcium alginate (CA) microsphere was prepared and used to construct a hydrogen peroxide (H2O2) biosensor by assembly with myoglobin (Mb) on the electrode surface. The elec-trochemical property of biosensor was investigated by cyclic voltammetry and chronoamperometry. In phosphate buffer solution (pH = 7) Mb-CA film modified electrode showed a pair of well-defined and nearly reversible cyclic voltammetry peaks at around ?0.358 V versus saturated calomel electrode (SCE). Moreover, the biosensors could still maintain their good activity and the sensitivity after 30 days. The results confirmed that the biosensors exhibit electrocatalytic behavior to H2O2. The biosensor with a linear range of from 6.25 μM to 50 μM (R2 = 0.996) and a detection limit of 1.25 μM (S/N = 3) showed good stability, reproducibility, and selectivity. The resulting biosensors are suitable for the precise determination of environmental pollutants in actual environment.

关 键 词：海藻酸钙 微球 肌红蛋白 电化学 电催化 

分 类 号：O65[理学—分析化学]