半滑舌鳎垂体细胞体外原代培养方法研究  

The Primary Culture Method on Pituitary Cells In Vitro of Half-Smooth Tongue Sole Cynoglossus semilaevis

作　　者：黄滨 王娜 史宝 丛林梅 陈松林 王蔚芳 

机构地区：[1]中国水产科学研究院黄海水产研究所,青岛市海水鱼类种子工程与生物技术重点实验室,山东青岛 [2]广州利洋水产科技股份有限公司,广东广州

出　　处：《水产研究》2017年第3期71-78,共8页Open Journal of Fisheries Research

基　　金：国家自然科学基金项目(31502177,31201982,31602133);国家鲆鲽类产业技术体(CARS-50);中国水产科学研究院基本科研业务费(2016GH03,2017HY-ZD0608)资助。

摘　　要：本文旨在探讨半滑舌鳎(Cynoglossus semilaevis)垂体细胞的体外原代培养方法。采用胰蛋白酶消化法,垂体细胞能够在含有5%的胎牛血清、100 U/mL青霉素和100 μg/mL链霉素的L-15完全培养基中,在24℃培养箱中,在不添加细胞生长因子的条件下培养7天以上,并分泌促性腺激素。结果显示,建立的垂体细胞原代培养方法适用于亲鱼和成鱼。体外原代培养的细胞既可以应用其开展相关的生殖生理研究,也可以从细胞层面探讨营养素发挥生殖调控的作用机制,为亲鱼营养调控机制研究提供便捷的实验材料。The primary culture method on pituitary cells in vitro of half smooth tongue sole (Cynoglossus semilaevis) was developed in this study. The cells were cultured in L-15 medium supplemented with 5% fetal bovine serum, 100 U/mL penicillin, 100 μg/mL streptomycin, and were survived as long as 7 days without cell growth factor in the incubator of 24?C. The cells can secrete the gonadotropin hormone. The results showed that this established method is suitable for both brood stock fish and adult fish. The cultured cells can be used in the research on reproductive physiology, also can be applied in the mechanism study on nutrition and reproduction at the cellular level with this convenient experimental material.

关 键 词：半滑舌鳎 垂体细胞 原代培养 营养与繁殖 

分 类 号：S9[农业科学—水产科学]