应用原位杂交技术检测感染鲤鱼体内的鲤春病毒  

Application of In Situ Hybridization in the Detection of Cyprinus carpio in Infected Carp

作　　者：吴斌[1] 王红[2] 张琳[1] 肇慧君[1] 

机构地区：[1]辽宁出入境检验检疫局,辽宁大连 [2]大连医科大学附属第一医院,辽宁大连

出　　处：《水产研究》2017年第3期92-98,共7页Open Journal of Fisheries Research

基　　金：国家质检公益项目(201410059)。

摘　　要：鲤春病毒(Spring Viremia of Carp Virus, SVCV)是一种弹状病毒,主要发生于春季,它能引起鲤科鱼类的鱼苗或成鱼大规模暴发鲤春病毒血症(SVC)。体外扩增的SVCV经人工方法感染健康鲤鱼,运用原位杂交技术(ISH),对感染后出现明显症状并经PCR鉴定阳性的病鱼,取其头部、内脏等组织切片进行SVCV分子定位和检测。结果显示,病鱼体内检出病毒,其中脑部和内脏含量较高。本文建立的SVCV原位杂交法,可初步确定SVCV在病鱼体内不同组织的分布情况。Spring Viremia of Carp Virus (SVCV) is a rhabdovirus that occurs mainly in the spring, which can cause large-scale outbreaks of Cyprinus carpio (falciparum) in fishes or adult fish. In vitro expansion of SVCV infected healthy carp by artificial infection. In situ hybridization (ISH) was used to identify and detect SVCV molecules in the head, viscera of diseased fish that with obvious symptoms of carp spring virulence and identified as positive by PCR. The results showed that there was a high level of virus in brain and viscera of diseased fish. In this study, SVCV in situ hybridization detection method was established, which can initially identify the distribution of SVCV in different tissues of diseased fish.

关 键 词：鲤春病毒(SVCV) 鲤春病毒血症(SVC) 鲤鱼 人工感染 原位杂交(ISH) 

分 类 号：S9[农业科学—水产科学]