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作 者:罗相忠[1] 童杰 梁宏伟[1] 李晓晖 邹桂伟[1] 崔峰
机构地区:[1]中国水产科学研究院长江水产研究所,湖北 武汉 [2]安徽科技学院生命与健康科学学院,安徽 凤阳
出 处:《水产研究》2023年第3期99-109,共11页Open Journal of Fisheries Research
摘 要:为研究麻醉剂MS-222对鲢的麻醉效果,比较分析了MS-222在高浓度分别为50、70、90、110、130 mg/L下诱导鲢进入各个阶段麻醉期以及复苏期的时间,及施麻后呼吸频率的变化。研究了10、20、30 mg/L MS-222低浓度下对鲢肝脏、脾脏、鳃组织的碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、过氧化氢酶(CAT)的活性影响。结果表明:MS-222溶液在高浓度条件下,110~130 mg/L为有效浓度,麻醉效果最好,适合对鲢进行短时间操作;在低浓度条件下,20~30 mg/L浓度试验组酶活高于对照组,且在一定时间内保持稳定,说明对鲢鱼种在长时间内保持麻醉效果,适宜对鲢进行长途运输。In order to analyze the anesthetic effect of the anesthetic MS-222 on silver carp, the time of MS-222 inducing silver carp to entering various stages of anesthesia and resuscitation at high concentrations of 50, 70, 90, 110, 130 mg/L were studied. Effects of alkaline phosphatase (AKP), acid phosphatase (ACP) and catalase (CAT) in liver, spleen and gill tissues of silver carp at low concentrations of 10, 20, 30 mg/L were observed. The results showed the anesthesia effect is the best at the effective concentration 110~130 mg/L under high concentration groups, which is suitable for short-term operations on silver carp, such as surgery. Under the concentration ranged from 20 to 30 mg/L, the enzyme activity of the experimental group is higher than that of the control group, which may remain stable for a certain period of time, such as fish transportation.
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