声光可调–近红外光谱技术即时快速检测川贝母中总生物碱含量方法研究  

A Real-Time Rapid Analysis Method for the Determination of Total Alkaloids in Fritillariae Cirrhosae Bulbus by AOTF-NIR

作　　者：武卫红 杨复森 孙岱 董海平[4] 

机构地区：[1]山东医学高等专科学校,山东 济南 [2]泰安市中心医院,山东 泰安 [3]山东省妇幼保健院,山东 济南 [4]济南金宏利实业有限公司,山东 济南

出　　处：《药物资讯》2020年第3期103-108,共6页Pharmacy Information

基　　金：山东省高等学校科技计划项目(J11LF28);山东省职业教育技艺技能传承创新平台资助项目(201712);山东省首届青年技能名师培养资助项目(2016).

摘　　要：目的:建立一种采用声光可调–近红外光谱(AOTF-NIR)技术即时快速检测川贝母中总生物碱含量的新方法。方法:依据2015年版《中国药典》川贝母项下方法,测定川贝母中总生物碱含量,利用偏最小二乘法(PLS1)建立校正集样本总生物碱含量测定值与其NIR光谱间的定量分析模型,并对验证集样本进行预测。结果:建立的总生物碱定量分析模型准确性较好,外部交叉验证均方差(RMSEP)为0.0022,决定系数(R2)为0.9831;所建模型对验证集样品的验证结果较好,平均相对误差为2.16%;预侧值与化学测定值间相关系数r为0.9909。结论:该方法快速、准确、无污染,可用于川贝母中总生物碱含量的即时快速检测分析。Objective: To establish a new method for real-time rapid determination of total alkaloids in Fritil-lariae Cirrhosae Bulbus by acousto-optic tunable filter-near infrared (AOTF-NIR) spectroscopy. Methods: The content of total alkaloids in the sample was measured with the 2015 edition of Chinese Pharmacopoeia. Then, multivariate calibration models based on PLS1 algorithm were developed to correlate the spectra and the corresponding values determined by the reference methods. Results: The quantitative analysis models of total alkaloids content performed well. RMSEP of the model was 0.0022 and the correlation coefficient of the models was 0.9909. The mean relative error for the test sets was 2.16%. Conclusions: The method is rapid, accurate and pollution-free, and can be used for the rapid determination of total alkaloids in Fritillariae Cirrhosae Bulbus.

关 键 词：声光可调近红外光谱技术 川贝母 生物碱 即时快速检测 

分 类 号：R28[医药卫生—中药学]