了哥王“汗渍法”炮制后对大鼠肝脏抗氧化能力的影响  

Effect of “Sweat Soaking Method” of the Wikstroemia indica on the Antioxidant Capacity of Rat Liver

作　　者：彭礼珍 冯果 李来来 许琴 李玮 任晨晨 朱光林 王文静 苏红梅 

机构地区：[1]贵州中医药大学,贵州 贵阳 [2]国家苗药工程技术研究中心,贵州 贵阳

出　　处：《药物资讯》2021年第6期365-371,共7页Pharmacy Information

摘　　要：目的:通过大鼠肝组织中抗氧化应激指标变化,分析“汗渍法”炮制了哥王前、后大鼠肝脏的抗氧化能力变化。方法:将健康SD雄性大鼠随机分为空白对照组、了哥王生品提取物高、中、低浓度组(0.4050、0.2025、0.1013 g/kg)、了哥王炮制品提取物高、中、低浓度组(0.4050、0.2025、0.1013 g/kg),每组6只。灌胃给药15天后,取肝脏,检测大鼠肝组织抗氧化相关因子超氧化物歧化酶(SOD)、丙二醛(MDA)、过氧化氢酶(CAT)、谷胱甘肽(GSH)含量或活性。结果:与空白对照组比较,给药了哥王生品组的SOD、CAT、GSH相对降低,MDA含量相对升高,炮制品组各指标无显著性差异。结论:了哥王经“汗渍法”炮制后,降低了药物对大鼠肝组织的毒性,修复大鼠肝脏抗氧化能力。Objective: Through the changes of the anti-oxidative stress indexes in the liver tissues of rats, the changes in the anti-oxidation ability of the livers of rats before and after processing by the “Sweat Soaking Method” of W. indica were analyzed Methods: Healthy male SD rats were randomly divided into a blank control group, a high, medium, and low concentration group of W. indica raw product ethanol extract (0.4050, 0.2025, 0.1013 g/kg), and a high, middle and low concentration group of W. indica raw product ethanol extract ( 0.4050, 0.2025, 0.1013 g/kg). After 15 days of intragastric administration, the liver was taken, and the antioxidant-related factors superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT) and glutathione (GSH) in rat liver tissue were detected. Results: Compared with the blank control group, the anti-oxidation test of rat liver tissue showed that the SOD, CAT, GSH of the W. indica raw material group were relatively lower, and the MDA content was relatively higher. There was no significant difference in the indicators of the processed product group. Conclusion: After being processed by the “Sweat Soaking Method” of W. indica, the toxicity of the drug to the liver tissue of rats is reduced, repairing the antioxidant capacity of rat liver.

关 键 词：了哥王 汗渍法 抗氧化 肝毒性 SD大鼠 

分 类 号：R28[医药卫生—中药学]