白芍药材及其配方颗粒的UPLC指纹图谱研究  

Study on UPLC Fingerprint of Paeonia lactiflora Pall. and Its Formula Granules

作　　者：刘红云 杨小松 吴红梅 王祥培 

机构地区：[1]贵州中医药大学药学院,贵州 贵阳 [2]贵州民族大学民族医药学院,贵州 贵阳

出　　处：《药物资讯》2023年第4期300-308,共9页Pharmacy Information

摘　　要：目的:建立白芍药材及其配方颗粒的UPLC指纹图谱。方法:色谱柱为ACQUITY UPLC BEH C18 (2.1 mm × 100 mm, 1.7 μm),以0.05%磷酸水溶液–乙腈为流动相,梯度洗脱,流速为0.1 mL/min;检测波长为254 nm;柱温为30℃;采用中药色谱指纹图谱评价系统进行相似度分析。结果:20批白芍药材及其配方颗粒的UPLC指纹图谱共确认11个共有峰,并指认出芍药苷和没食子酸两种化学成分。结论:该方法准确、可行、重现性好,反映白芍药材及配方颗粒多成分的整体面貌,可为白芍药材及其配方颗粒质量评价提供参考。Objective: To establish the UPLC fingerprint of Paeonia lactiflora Pall. and its formula granules. Methods: The chromatographic column is ACQUITY UPLC BEH C18 (2.1 mm × 100 mm, 1.7 μm) with acetonitrile-0.05% phosphoric acid aqueous solution as the mobile phase in a gradient mode at the flow rate of 0.1 mL/min. The detection wavelength is 254 nm and the column temperature is 30˚C. The similarity is evaluated using a traditional Chinese medicine chromatographic fingerprint eval-uation system. Results: A total of 11 common peaks were identified in the UPLC fingerprint of 20 batches of Paeonia lactiflora Pall. and their formula granules, and two chemical components, pae-oniflorin and gallic acid, were identified. Conclusion: This method is accurate, feasible, and has good reproducibility, reflecting the overall appearance of multiple components in Paeonia lactiflora Pall. and its formula granules. It can provide a reference for the quality evaluation of the Paeonia lactiflora Pall. and its formula granules.

关 键 词：白芍 UPLC指纹图谱 配方颗粒 

分 类 号：R28[医药卫生—中药学]