紫草药材及其配方颗粒的薄层色谱研究  

Study on Thin Layer Chromatography of Arnebiae Radix herb and Its Formula Granules

作　　者：刘梅 王祥培 张朝尔 吴红梅 

机构地区：[1]贵州中医药大学药学院,贵州 贵阳 [2]贵州民族大学民族医药学院,贵州 贵阳

出　　处：《药物资讯》2023年第4期359-369,共11页Pharmacy Information

摘　　要：目的:对紫草药材显微结构特征进行鉴定及对药材与其配方颗粒的薄层色谱进行研究,为紫草及其配方颗粒质量控制的均一性、稳定性提供参考依据。方法:参照2020版《中国药典》标准对紫草药材进行显微鉴定。此外,参照现行药典紫草鉴别项下薄层色谱方法,对配方颗粒前处理提取溶剂进行优选,并对薄层色谱条件进行温度、湿度考察。结果:紫草药材的显微特征,均符合药典标准。薄层色谱结果显示,按现行药典紫草鉴别项下薄层色谱法,紫草药材有7个斑点,但其配方颗粒没有斑点。优选提取溶剂后,紫草药材有7个斑点,配方颗粒有1个斑点,两者均有相同斑点1个。结论:薄层色谱法能阐明紫草药材及配方颗粒薄层色谱两者成分的一致性及差异性问题,可为紫草药材及其配方颗粒质量的均一性、稳定性评价方法提供参考。Purpose: Identification of the microstructure characteristics of Arnebiae Radix medicinal herb and study on the thin layer chromatography of the medicinal herb and its formula particles provide a reference basis for the uniformity and stability of quality control of Arnebiae Radix and its formula particles. Method: Microscopic identification of Arnebiae Radix herb was conducted according to the 2020 edition of the Chinese Pharmacopoeia. In addition, referring to the thin layer chromatography method under the current pharmacopoeia identification of Arnebiae Radix, the extraction solvent for the pre-treatment of formula particles was optimized, and the temperature and humidity of the thin layer chromatography conditions were investigated. Result: The microscopic characteristics of Arnebiae Radix medicinal herbs all comply with pharmacopoeia standards. The results of thin layer chromatography show that according to the current pharmacopoeia’s identification of Arnebiae Radix, there are 7 spots on the purple grass medicinal material, but the formula particles did not have any spots. After selecting the optimal extraction solvent, the Arnebiae Radix herb has 7 spots, the formula particles have 1 spot, and both have the common 1 spot. Conclusion: Thin layer chroma-tography can elucidate the consistency and differences between the components of Arnebiae Radix medicinal herb and formula granules in thin layer chromatography. This can provide a reference for evaluating the uniformity and stability of the quality of Arnebiae Radix medicinal herb and its formula granules.

关 键 词：紫草 配方颗粒 薄层色谱 显微鉴别 

分 类 号：R28[医药卫生—中药学]