太子参不同极性部位的化学成分研究  

Study of Chemical Constituents of Different Polarity Fractions from Pseudostellaria heterophylla

作　　者：阚永军 刘莹莹[2] 赵立[2] 蒋畅[2] 庞文生[3] 胡娟 

机构地区：[1]福建省中医药科学院,福建 福州 [2]福建中医药大学附属第二人民医院,福建 福州 [3]福建中医药大学药学院,福建 福州

出　　处：《药物资讯》2023年第6期512-516,共5页Pharmacy Information

摘　　要：目的:对太子参不同极性部位中的化学成分进行鉴定,并比较不同极性部位之间化学成分的差异。方法:将太子参饮片用80%乙醇制备总提取物后,分别用乙酸乙酯和正丁醇依次萃取,得到不同极性部位提取物。采用InfiniteC18色谱柱(4.6 mm × 250 mm, 5 μm),以乙腈(A)和0.2%甲酸溶液(B)为流动性进行梯度洗脱,流速为0.3 mL•min−1;离子源采用电喷雾离子源(ESI),扫描模型是正离子模式,结合Metlin数据库和文献报道的质谱信息对太子参不同极性部位中的化学成分进行鉴定。结果:从太子参乙酸乙酯和正丁醇部位中分别鉴定出10个和7个化合物。结论:本研究为太子参的药效物质基础研究提供了理论参考。Objective: To identify the chemical components in different polarity fractions of Pseudostellaria heterophylla and compare the differences in chemical components between different polarity frac-tions. Methods: The Pseudostellaria heterophylla were prepared with 80% ethanol to obtain the total extract, and then sequentially extracted with ethyl acetate and n-butanol to obtain the extracts of different polarity fractions. A InfiniteC18 column (4.6 mm × 250 mm, 5 μm) was used with ace-tonitrile (A) and 0.2% formic acid solution (B) as the mobile phase for gradient elution at a flow rate of 0.3 mL•min−1. The ion source was an electrospray ionization (ESI), and the scanning model was positive ion mode. The chemical components in different polarity parts of Pseudostellaria heterophylla were identified by combining the mass spectrometry information from the Metlin database and literature reports. Results: Ten and seven compounds were identified from the ethyl acetate and n-butanol parts of Pseudostellaria heterophylla, respectively. Conclusion: This study provided a theoretical reference for the study of the pharmacological substance basis of Pseudostellaria heterophylla.

关 键 词：太子参 不同极性部位 化学成分 

分 类 号：R28[医药卫生—中药学]