水母胶原联合SOD对骨质疏松症模型小鼠的干预作用  

The Interventional Effect of Jellyfish Collagen Combined with SOD on a Mice Model of Osteoporosis

作　　者：刘倩 陈永 唐中生 徐蕾 阮仁全 

机构地区：[1]贵州中医药大学基础医学院,贵州 贵阳 [2]遵义医科大学附属医院风湿免疫科,贵州 遵义 [3]贵州中医药大学解剖学教研室,贵州 贵阳 [4]美尔健(深圳)生物科技有限公司研发部,广东 深圳 [5]美尔健(深圳)生物科技有限公司总经理办公室,广东 深圳

出　　处：《药物资讯》2024年第4期341-347,共7页Pharmacy Information

摘　　要：目的:观察水母胶原联合超氧化物歧化酶(JCSOD)对糖皮质激素诱导的骨质疏松症模型小鼠的干预作用。方法:将C57BL/6小鼠分为正常对照组(NC)、模型组(Dex)、JCSOD低剂量组(JCSOD-L)、JCSOD高剂量组(JCSOD-H)、水母胶原组(JC)、氨糖软骨素钙片组(ACc),每组5只。干预60天,结束后采集骨组织和血清,分别进行病理组织学和骨胶原纤维形态学检测,采用酶联免疫吸附法(ELISA)进行骨特异性碱性磷酸酶(BALP)和活性氧(ROS)含量检测。结果:病理组织学检测和骨胶原纤维形态学检测提示,与Dex组相比,JCSOD-H组和JC组显著维持了骨小梁的构架,提高了骨胶原的着色深度和纹理的清晰度,JCSOD-L组和ACc组效果较差,但效果优于Dex组。ELISA法检测显示,与Dex组相比,JCSOD低、高剂量组、JC组和ACc组都显著提高了血清BALP的含量(P < 0.005),降低了骨组织ROS的含量(P < 0.005)。结论:JCSOD可以通过保持骨小梁的架构、提高骨胶原纤维的染色深度、增加BALP的表达和减少ROS的含量来减缓激素诱导的骨质疏松症小鼠的病情进展。Objective: To observe the intervention effect of jellyfish collagen combined with superoxide dismutase (JCSOD) on glucocorticoid-induced osteoporosis model mice. Methods: C57BL/6 mice were divided into normal control group (NC), model group (Dex), JCSOD low-dose group (JCSOD-L), JCSOD high-dose group (JCSOD-H), jellyfish collagen group (JC) and aminosaccharide chondroitin calcium tablet group (ACc), with 5 mice in each group. After 60 days of intervention, bone tissue and serum were collected, and histopathological and bone fibrillar morphology were detected, respectively. Bone-specific alkaline phosphatase (BALP) and reactive oxygen species (ROS) contents were detected by enzyme-related immunosorbent assay (ELISA). Results: Compared with Dex group, JCSOD-H and JC groups significantly maintained bone trabecular structure and improved the depth of collagen staining and texture clarity. JCSOD-L and ACc groups had worse effects, but had better effects than Dex group. ELISA assay showed that compared with Dex group, JCSOD low, high dose, JC and ACc groups significantly increased serum BALP content (P < 0.005), and decreased bone tissue ROS content (P < 0.005). Conclusion: JCSOD can slow down the progression of steroid-induced osteoporosis in mice by maintaining the structure of bone trabeculae, increasing the staining depth of bone fibrils, increasing the expression of BALP and decreasing the content of ROS.

关 键 词：骨质疏松症 糖皮质激素 水母胶原 骨特异性碱性磷酸酶 活性氧 

分 类 号：R58[医药卫生—内分泌]