甘草提取液和海尔福清千口服液对铅染毒小鼠的干预效果的试验  

The Therapeutic Effect of Licorice Extract and Qingqian Oral Liquid on Lead Poisoning Mice

作　　者：蒙宏杰 杨梅春 赵小秋 梅金钰 张树球 龙奇军[1] 

机构地区：[1]右江民族医学院重金属与氟砷毒物研究实验室,广西 百色 [2]右江民族医学院附属医院,广西 百色 [3]广西云球生物科技有限公司,广西 百色

出　　处：《中医学》2021年第1期114-120,共7页Traditional Chinese Medicine

摘　　要：目的:研究甘草提取液和海尔福清千口服液(原海尔福口服液)对铅中毒小鼠的治疗效果。方法:提取、制备甘草提取液、海尔福清千口服液,造模前取血测定血红蛋白(Hb);铅中毒模型的建立:除正常组外,其余各组用4 mg/ml乙酸铅溶液按14 mg/kg给小鼠腹腔注射,每日1次,直到实验结束;当造模2周后,治疗1组(甘草组)用甘草提取液0.2 ml/只,经口灌胃给小鼠灌服,每日一次至实验结束。治疗2组(清千组)用海尔福清千口服液0.2 ml/只,经口灌胃,给小鼠灌服,每天一次,连续二周。实验对照组(模型组)和正常对照组用相同量体积的纯净水灌胃;试验最后,抽取小鼠全血,静置,待血液凝固析出血清,离心,取血清测定GPT、GC、TC、尿素等;处死动物后取器、用甲醛浸泡后,制作切片,做镜下病理研究;取大脑,在冷浴下用0.9%氯化钠盐水制成10%脑匀浆,测定大脑各种指标,如 清除率。结果:正常组、模型组、甘草组、清千组测定结果依次排列;染毒前Hb分别为176.65 ±13.32、181.73 ±14.76、183.48 ±19.30、196.04 ±13.97 (g/L),四组间比较分析,差异明显,有显著性;灌药前(造模后) Hb分别为175.88 ±16.95、119.75 ±16.99、123.23 ±15.35、115.46 ±24.14 (g/L),染毒各组明显低于正常组,P 【0.01,染毒组明显下降;灌药后Hb分别为177.51 ±10.30、121.12 ±27.43、149.56 ±9.75、152.04 ±11.46 (g/L),染毒各组仍低于正常组,P 【0.01,但灌药组已有明显上升,接近正常;TG分别为0.75 ±0.15、1.22 ±0.63、0.76 ±0.20、0.56 ±0.17 (mmol),四组比较,差异明显,有统计学意义;TC分别为4.88 ±0.31、6.10 ±0.69、4.74 ±1.03、4.17 ±0.82 mmol四组比较,差异明显,有统计学意义;尿素氮分别为6.54 ±0.91、8.09 ±1.41、5.74 ±1.21、6.02 ±1.99 mmol,四组比较,差异明显,有统计学意义;血清GPT活力分别为8.00 ±3.56、10.50 ±5.3Objective: To study the therapeutic effect of licorice extract and Haerfuqing Thousand Oral liquid (original Haerfuqing oral liquid) on lead poisoning mice. Methods: Extract and prepare Glycyrrhiza uralensis extract and Hailfuqing Thousand Oral liquid, and take blood for hemoglobin (Hb) before modeling. Establishment of lead poisoning model: Except the normal group, the mice in other groups were intraperitoneally injected with 4 mg/ml lead acetate solution at 14 mg/kg, once a day until the end of the experiment. After 2 weeks of modeling, the treatment group 1 (the Glycyrrhiza uralensis group) was given 0.2 ml of Glycyrrhiza uralensis extract through oral gavage to mice, once a day until the end of the experiment. The treatment group 2 (the Qingqian group) was orally administrated with 0.2 ml of Hailfuqingqian oral solution to mice once a day until the end of the experiment. In the model group and the normal group, equal volume distilled water was used instead of irrigation, and the method was the same. At the end of the experiment, blood was collected from the eyeball of mice, serum was separated and ALT and urea were measured. After the animals were killed, the liver and kidney were taken and fixed with 10% formaldehyde, and the slices were made for pathological study under the microscope. The brain was taken and frozen to make 10% brain homogenate with normal saline to determine the free radical scavenging rate of brain. Results: The results of the normal group, the model group, the Licorice group and the Qingqian group were arranged in order. Before modeling, Hb was 176.65 ±13.32, 181.73 ±14.76, 183.48 ±19.30 and 196.04 ±13.97 (g/L), respectively. The difference of hemoglobin (Hb) between groups was statistically significant. After modeling (before administration), Hb was 175.88 ±16.95, 119.75 ±16.99, 123.23 ±15.35, 115.46 ±24.14 (g/L), which was significantly higher in the normal group than in other groups, P

关 键 词：铅染毒 小白鼠 海尔福清千口服液 甘草提取液 灌药 

分 类 号：R28[医药卫生—中药学]