热毒宁、甘草酸苷联合治疗对II期手足口病患儿免疫细胞及炎性细胞因子的影响  

Effect of Reduning Combined with Glycyrrhizin on Immune Cells and Inflammatory Cytokines of Children with the Second Stage of Hand Foot and Mouth Disease

作　　者：黄胜贤[1] 罗杰平[1] 陈丽珍[1] 邓少芳 黄庆华 叶潇鸣 

机构地区：[1]南宁市第二人民医院,广西 南宁

出　　处：《中医学》2021年第1期132-137,共6页Traditional Chinese Medicine

摘　　要：目的:探讨热毒宁、甘草酸苷联合治疗对II期手足口病患儿的免疫细胞及炎性细胞因子的影响。方法:286例II期手足口病患儿随机分成实验1组(66例)、实验2组(73例)、实验3组(72例)及实验4组(75例),同期63例健康体检儿童作为对照组。应用流式细胞术检测所有研究对象外周血T淋巴细胞、Th细胞、Tc细胞、NK细胞和B淋巴细胞所占淋巴细胞的百分比。酶联免疫吸附法测定炎性细胞因子IL-6、IL-10.、TNF-ɑ水平,分析各组间上述指标的差异。结果:1) II期手足口病患儿T淋巴细胞、Th细胞、Th/Tc细胞比值较对照组显著降低,而B淋巴细胞百分比、IL-6、IL-10和TNF-ɑ水平明显升高,差异有统计学意义(P 【0.05);2) 较实验1组,实验2组、实验3组及实验4组的T淋巴细胞、Th细胞、Th/Tc比值显著升高,B淋巴细胞及IL-6、IL-10.、TNF-ɑ的水平明显降低(P皆 【0.05);且实验2组与实验4组的Th细胞、B淋巴细胞、Th/Tc比值、IL-6、IL-10.、TNF-ɑ间的差异有统计学意义(P 【0.05)。结论:较单用热毒宁,热毒宁及甘草酸苷联合治疗能显著地缓解II期手足口患儿的免疫功能紊乱,但热毒宁联合甘草酸苷改善免疫功能的作用较单独用药是否有叠加作用需进一步研究。Objective: To investigate the effect of Reduning combined with glycyrrhizin on immune cells and inflammatory cytokines in children with hand-foot-mouth disease (HFMD). Methods: 286 children with Stage II HFMD were randomly divided into the routine group (66 cases), the Reduning group (73 cases), the glycyrrhizin group (72 cases) and the combined group (75 cases), and 63 healthy children as the control group. The percentage of T lymphocyte, Th cells, Tc cells, NK cells, and B lymphocyte in the lymphocyte of all subjects’ peripheral blood was measured by flow cytometry. Inflammatory cytokines IL-6, IL-10 and TNF-ɑ were measured by ELISA, to analyze the differences of the above indicators among the groups. Results: 1) The ratio of T lymphocyte cells, Th cells and Th/Tc cells was significantly lower than that of the control group, but the percentage of B lymphocyte, IL-6, IL-10 and TNF-ɑ levels was significantly higher than that of the control group (P

关 键 词：热毒宁 甘草酸苷 手足口病 免疫细胞 炎性细胞因子 

分 类 号：R28[医药卫生—中药学]