灵芝多糖对精子膜及DNA作用的研究  

Effect of Polysaccharide from Ganoderma lucidum on Sperm Membrane and DNA Integrity

作　　者：房振亚 张美华 李安娜 马彦超[2] 

机构地区：[1]国家卫健委生育调控技术重点实验室,山东省妇幼保健院,山东 济南 [2]菏泽市立医院妇产科,山东 菏泽

出　　处：《中医学》2021年第3期358-363,共6页Traditional Chinese Medicine

摘　　要：目的:主要针对灵芝多糖的针对性疗效展开了细化的分析,据此尝试对其对于精子膜和DNA完整性的影响形成全面而深刻的认识,从而在临床上为少弱精子症提供针对性的治疗方案。方法:为实现这一研究目的,本次实验的开展主要选取了正常的成年男性,取其精液进行对照比较,形成对照的主要有等量的生理盐水及不同浓度灵芝多糖溶液(0.25, 1.0, 5.0 mg/ml)。通过伊红–Y染色试验、精子低渗肿胀实验(HOS)以及精子染色质扩散(SCD)实验,针对不同浓度的灵芝多糖所产生的影响展开了细化的研究与探讨,试图了解到其对精子膜以及DNA实际产生的作用力情况表现。结果:从结果表现来看,不同浓度的灵芝多糖都可以对精子的存活时间起到拉长效果,从而使得精子的存活率因此得到切实的提升,并且在这一过程中切实使得精子膜和DNA得到不同程度的保护功效。从具体对比分析来看,中高灵芝多糖起到的作用效果明显更高,呈现出更为优秀的结果表现。结论:灵芝多糖有体外提高精子存活率和保护精子 DNA完整性的作用。Objective: To observe the effect of Ganoderma lucidum polysaccharides on sperm membrane and DNA integrity in vitro. It provides a new way for the treatment of oligoasthenospermia. Methods: Normal adult male, abstinence 3 - 5 d masturbation method semen. They were divided into 5 groups, and 0.2 ml semen was collected from each group. The control group was semen stock, and the other 4 groups were added with equal amounts of normal saline, and ganoderma polysaccharides solution of different concentrations (0.25, 1.0, 5.0 mg/ml). The effects of different concentrations of Ganoderma lucidum polysaccharides on sperm survival rate, sperm membrane integrity and sperm nucleus DNA were observed by eosin-Y staining, HOS and SCD. Results: Under the same conditions, low, medium and high concentrations of Ganoderma lucidum polysaccharides could increase sperm survival time, improve sperm survival rate and motility, reduce sperm DNA damage rate, and protect sperm membrane function and DNA. The effect of polysaccharides in the 1.0 mg/ml and 5.0 mg/ml (medium and high) Ganoderma lucidum group was significantly better than that in the control group (P <0.05 or P <0.01). Conclusion: Ganoderma lucidum polysaccharides can improve sperm survival rate and protect sperm DNA integrity in vitro.

关 键 词：灵芝多糖 精子 体外 DNA完整性 

分 类 号：R28[医药卫生—中药学]