基于网络药理学的丹芪通络方治疗颈椎病作用机制研究  

Study on the Mechanism of Danqi Tongluo Recipe in Treating Cervical Spondylosis Based on Network Pharmacology

作　　者：张宛宜 曾怡 吕虹宇 孙德利[2] 

机构地区：[1]上海中医药大学研究生院,上海 [2]上海中医药大学附属龙华医院针灸科,上海 [3]上海中医药大学研究生院,上海

出　　处：《中医学》2022年第2期213-223,共11页Traditional Chinese Medicine

摘　　要：目的:基于网络药理学方法探讨丹芪通络方治疗颈椎病的潜在作用机制。方法:采用TCMSP数据库筛选出丹芪通络方的活性成分,使用Uni Prot系统预测及规范活性成分的潜在靶点;使用Genecards、OMIM、Disgenet数据库检索颈椎病和颈椎综合征的相关靶点;使用Cytoscape软件构建出药物与疾病靶点的蛋白质相互作用网络,使用Merge功能提取二者的交集网络,交集网络可能就是丹芪通络方治疗颈椎病的关键靶点;使用Metascape网站对上述提取出的关键靶点进行GO和KEGG富集分析。结果:获得有效成分226个,药物靶点299个,颈椎病相关靶点6704个,最终得到关键靶点146个、KEGG通路364条。丹芪通络方治疗颈椎病主要成分可能为槲皮素、β-谷甾醇、植物甾醇、山柰酚、木犀草素等,涉及到的关键通路有癌症信号通路、IL-17信号通路、HIF-1信号通路、MAPK信号通路、p53信号通路等。结论:本项研究初步揭示了丹芪通络方多成分、多靶点、多途径治疗颈椎病的机制,为临床治疗颈椎病提供了新方法。Objective: To explore potential mechanism of Danqi Tongluo Recipe in treating cervical spondylosis based on network pharmacological method. Methods: Based on the TCMSP Chinese medicine system pharmacology database and analysis platform, the active components of Danqi Tongluo Recipe were obtained, and the target gene was predicted according to the Uni Prot system. The targets of cervical spondylosis and cervicobrachial syndrome were searched through databases of Genecards, OMIM and Disgenet. Cytoscape software was used to establish protein-protein interaction (PPI) networks of medicinal substances of Danqi Tongluo Recipe and cervical spondylosis. Enrichment analyses of GO and KEGG on the key targets were performed by Metascape. Results: There were 226 medicinal components, 299 medicinal targets, 6704 targets for cervical spondylosis, 146 key targets and 364 KEGG pathways. The main components of Danqi Tongluo Recipe in treating cervical spondylosis were quercetin, beta-sitosterol, stigmasterol, sitosterol, kaempferol, luteolin, etc. The key targets were HSPA8, RPS27A, RPS27, STAU1, etc. The key biological processes and pathways might include signaling pathways of pathways in cancer, IL-17 signaling pathway, HIF-1 signaling pathway, MAPK signaling pathway, p53 signaling pathway, etc. Conclusion: This study preliminarily reveals a multiple component, target and pathway mechanism of Danqi Tongluo Recipe in treating cervical spondylosis. It provides a new idea for clinical drug prescriptions of cervical spondylosis.

关 键 词：网络药理学 丹芪通络方 颈椎病 信号通路 

分 类 号：R285[医药卫生—中药学]