基于网络药理学的丹芪通络方治疗腰椎间盘突出症作用机制研究  

Study on the Mechanism of Danqi Tongluo Recipe in Treating Lumbar Disc Herniation Based on Network Pharmacology

作　　者：曾怡 张宛宜 吕虹宇 孙德利[2] 

机构地区：[1]上海中医药大学研究生院,上海 [2]上海中医药大学附属龙华医院,上海

出　　处：《中医学》2022年第3期407-415,共9页Traditional Chinese Medicine

摘　　要：目的:基于网络药理学方法探讨丹芪通络方治疗腰椎间盘突出症的潜在作用机制。方法:在TCMSP数据库筛检索出丹芪通络方的活性成分及活性成分靶点。在Genecards、OMIM、Disgenet数据库检索腰椎间盘突出症的相关靶点;利用Venny图得出丹芪通络方与腰椎间盘突出共同靶点;利用Cytoscape构建药物与疾病靶点的蛋白质相互作用网络,并提取交集网络以获得丹芪通络方和腰椎间盘突出症的共同相关靶点;利用Metascape对共同相关靶点进行GO和KEGG富集分析。结果:得到药效成分226个、药物靶点242个、腰椎间盘突出症靶点2414个,其中药物与疾病共同靶点125个。丹芪通络方治疗腰椎间盘突出症涉及到的成分有挥发油、菊糖、阿魏酸、谷甾醇类、植物甾醇、山柰酚、槲皮素、丹参酮、酚酸类等,涉及到的关键通路有eb病毒感染、癌症信号通路、MAPK信号通路、WNT信号通路、甲状腺激素信号通路、泛素介导的蛋白质水解过程等,与泛素样蛋白连接酶结合、激酶结合、转录因子结合、mRNA分解代谢过程、细胞蛋白分解过程、DNA修复、蛋白质结构域特异性结合、细胞粘附分子结合、端粒组织、细胞蛋白定位的调控等多个生物学过程相关。结论:本研究基于网络药理学上初步揭示了丹芪通络方治疗腰椎间盘突出症的潜在机制。Objective: To explore the potential mechanism of Danqi Tongluo Recipe in the treatment of lum-bar disc herniation based on network pharmacology. Methods: The active components and active component targets of Danqi Tongluo Recipe were screened in TCMSP database. The relevant targets of lumbar disc herniation were searched in Genecards, OMIM, Disgenet databases. The common target of Danqi Tongluo Recipe and lumbar disc herniation was obtained by Venny diagram. The protein interaction network between drugs and disease targets was constructed by Cytoscape, and the intersection network was extracted to obtain the common related targets of Danqi Tongluo Recipe and lumbar disc herniation. GO and KEGG enrichment analysis of common related targets was carried out by Metascape. Results: 226 effective components, 242 drug targets and 2414 targets of lumbar disc herniation were obtained, including 125 common targets of drugs and diseases. The components involved in the treatment of lumbar disc herniation by Danqi Tongluo Recipe include volatile oil, inulin, ferulic acid, sitosterol, phytosterol, kaempferol, quercetin, tanshinone, phenolic acids, etc. The key pathways involved include EB virus infection, Pathways in cancer, MAPK signaling pathway, Wnt signaling pathway, thyroid hormone signal pathway, ubiquitin mediated protein hydrolysis process, etc., which bind to ubiquitin like protein ligase, kinase Transcription factor binding, mRNA catabolism, cellular proteolysis, DNA repair, protein domain specific binding, cell adhesion molecule binding, telomere tissue, regulation of cellular protein localization and other biological processes. Conclusion: Based on network pharmacology, this essay preliminarily reveals the potential mechanism of Danqi Tongluo Recipe in the treatment of lumbar disc herniation.

关 键 词：网络药理学 丹芪通络方 腰椎间盘突出症 蛋白靶点 生物过程 信号通路 

分 类 号：R285[医药卫生—中药学]