生酮饮食治疗脊髓损伤关键基因的筛选及潜在中药预测  

Selection of Key Genes for Ketogenic Diet Treatment of Spinal Cord Injury and Prediction of Potential Chinese Herbal Medicines

作　　者：李素福 祁文[2] 梁永健 李哲榕 

机构地区：[1]广西中医药大学附属瑞康临床医学院,广西 南宁 [2]广西中医药大学研究生院,广西 南宁

出　　处：《中医学》2024年第1期69-77,共9页Traditional Chinese Medicine

摘　　要：目的:利用生物信息学技术分析生酮饮食对治疗脊髓损伤的潜在分子靶点和相关通路,同时通过靶基因预测潜在的中药药物用于治疗脊髓损伤。在GEO数据库中提取与脊髓损伤治疗相关的生酮饮食芯片组数据,并利用R语言软件对芯片数据进行分析,找出差异表达的基因。然后对这些差异基因进行功能富集分析,以获取与差异基因相关的信号通路和分子功能。并构建基因–蛋白网络,然后使用Cytoscape对该网络进行分析,以获取治疗脊髓损伤的关键基因。接着将这些基因映射到CTD数据库获得的脊髓损伤疾病基因网络中,以确定生酮饮食治疗脊髓损伤过程中的核心基因。最后,通过将核心基因与医学本体信息检索平台(Coremine Medical)相互映射,筛选出治疗脊髓损伤的中药。结果:GEO芯片组数据分析得出生酮饮食治疗脊髓损伤的差异基因有237个,最后筛选出10个核心基因包括LCN2、TTR、ORM1、ATP6V0D2、TNS1、HTR2C、ACTRT2、UOX、KCNAB3、RAB9B。治疗脊髓损伤的潜在中药药物有:大黄、三七、黄芪、充蔚子。Objective: To analyze potential molecular targets and related pathways of ketogenic diet in the treatment of spinal cord injury using bioinformatics techniques, and predict potential Chinese herbal medicines for the treatment of spinal cord injury through target gene analysis. Method: Mi-croarray data of ketogenic diet treatment for spinal cord injury in the GEO database were extracted, and differentially expressed genes were obtained through analysis using R software. Functional en-richment analysis was performed on the differentially expressed genes to identify related signaling pathways and molecular functions. Using STING, a protein-protein interaction network of the genes was constructed, and Cytoscape was used to analyze the network and identify key genes in the treatment of spinal cord injury. The identified genes were mapped to the spinal cord injury disease gene network obtained from the CTD database, and core genes of the ketogenic diet treatment pro-cess for spinal cord injury were obtained. Finally, using Coremine Medical, the core genes were mapped to the medical ontology information retrieval platform to screen potential Chinese herbal medicines for the treatment of spinal cord injury. Results: Analysis of GEO microarray data revealed 237 differentially expressed genes associated with ketogenic diet treatment for spinal cord injury. Ten core genes, including LCN2, TTR, ORM1, ATP6V0D2, TNS1, HTR2C, ACTRT2, UOX, KCNAB3 and RAB9B, were selected. Potential Chinese herbal medicines for the treatment of spinal cord injury included Rhubarb, Sanqi, Astragalus and Chongweizi.

关 键 词：生物信息学 脊髓损伤 生酮饮食 分子机制 蛋白互作网络 神经保护 神经修复 

分 类 号：TP3[自动化与计算机技术—计算机科学与技术]