怀山药提取物DM-2治疗C-BSA诱导的慢性肾炎的实验研究  

Experimental Study on the Treatment of Dioscorea opposita Thunb. Extract DM-2 on C-BSA Induced Chronic Nephritis Rat Model

作　　者：多力坤·马木特 玛伊热·阿卜杜克热木 伊克拉木·伊力哈木 买吾拉尼江·依孜布拉 

机构地区：[1]新疆沙乐美生物科技有限公司,新疆 乌鲁木齐 [2]新疆医科大学临床医学部,新疆 乌鲁木齐 [3]新疆医科大学中心实验室,新疆 乌鲁木齐

出　　处：《中医学》2024年第3期500-508,共9页Traditional Chinese Medicine

摘　　要：目的:研究怀山药提取物DM-2对阳离子化牛血清白蛋白(C-BSA)诱导的慢性肾炎模型大鼠的治疗作用。方法:SD大鼠70只,雄性,随机分为正常对照组、慢性肾炎模型组、雷公藤多苷阳性对照组(6.5 mg/kg)、醋酸泼尼松阳性对照组(8.0 mg/kg)以及怀山药提取物DM-2低、中、高剂量(1.0, 2.0, 4.0 g/kg)给药组;采用C-BSA制备肾炎模型大鼠,用怀山药提取物DM-2进行药物干预4周,检测DM-2对肾炎模型大鼠24 h尿蛋白量水平的影响;检测血清总蛋白、胆固醇白蛋白及血清肌酐和尿素氮的含量的影响,同时通过HE染色方法观察DM-2对肾炎模型大鼠肾脏组织病例形态的影响。结果:C-BSA诱导肾炎模型大鼠尿蛋白以及血清肌酐和尿素氮显著增加(P P P P < 0.05)。HE染色结果显示,DM-2中、高剂量和醋酸泼尼松给药组大鼠肾脏病变病理特征显著减轻。结论:怀山药提取物DM-2对C-BSA诱导的慢性肾炎大鼠具有良好的治疗作用,能够成为治疗慢性肾炎的候选植物药。Objective: To study the therapeutic effect of Dioscorea opposita Thunb. extract DM-2 on cationized bovine serum albumin (C-BSA) induced chronic nephritis rat model. Methods: 70 SD male rats were randomly divided into normal control group, chronic nephritis model group, tripterygium glycoside positive control group (6.5 mg/kg), prednisone acetate positive control group (8.0 mg/kg) and DM-2 low, medium and high dose groups (1.0, 2.0, 4.0 g/kg). C-BSA was used to prepare nephritis model rats, all administration groups received relevant medicine for consuccessive 4 weeks. The effect of DM-2 on 24 h urinary protein level of nephritis model rats was detected. The contents of serum total protein, cholesterol albumin, serum creatinine and urea nitrogen were detected, effects of DM-2 on the morphology of renal tissue in rats with nephritis were observed by HE staining method. Results: Urinary protein, serum creatinine and urea nitrogen level were significantly increased (P P P P < 0.05). HE staining results showed that the pathological features of renal lesions were significantly reduced in DM-2 medium, high dose and prednisone acetate groups. Conclusion: DM-2 has a good therapeutic effect on C-BSA-induced chronic nephritis in rats, and can be a candidate plant drug for chronic nephritis.

关 键 词：怀山药 慢性肾炎 阳离子化牛血清白蛋白 

分 类 号：R28[医药卫生—中药学]