多指标综合加权评分法结合Box-Behnken响应面法优选葛根芩连汤醇提工艺  

The Alcohol Extraction Process of Gegen Qinlian Decoction Was Optimized by Multi-Index Comprehensive Weighted Scoring Method Combined with Box-Behnken Response Surface Method

作　　者：秦琴 刘文 宋信莉 刘巍 

机构地区：[1]贵州中医药大学药学院,国家苗药工程技术研究中心,贵州中药炮制与制剂工程技术研究中心,贵州 贵阳 [2]贵州医科大学药学院,贵州 贵阳

出　　处：《中医学》2024年第8期1803-1821,共19页Traditional Chinese Medicine

摘　　要：目的:采用多指标综合加权评分法结合Box-Behnken响应面法优选葛根芩连汤的提取工艺。方法:以前期通过谱效关系筛选的有效成分葛根素、大豆苷、黄连碱、表小檗碱、盐酸药根碱、盐酸小檗碱、盐酸巴马汀、黄芩苷、汉黄芩苷和甘草酸铵为考察指标,采用高效液相色谱法建立葛根芩连汤提取物中10种成分的含量测定方法,并对其进行综合加权评分。采用单因素试验考察提取方法、乙醇浓度、提取时间、提取次数和溶剂倍数对葛根芩连汤有效成分的影响,结合Box-Behnken响应面法,以综合加权评分值为评价指标,优化葛根芩连汤的最佳提取工艺。结果:优选出葛根芩连汤最佳提取工艺为药材用8倍量的85.5%乙醇,提取2.5 h,提取次数2次。验证试验表明该条件下各指标成分含量、浸膏得率较高,且RSD值均小3%。结论:优选的葛根芩连汤提取工艺稳定可行、重现性良好,可为其成型工艺研究提供一定实验基础和参考依据。OBJECTIVE: Multi-index comprehensive weighted scoring method combined with Box-Behnken response surface method was used to optimize the extraction process of Gegen Qinlian decoction. METHODS: Using puerarin, daidzein, coptisine, epicberberine, rhizarine hydrochloride, berberine hydrochloride, palmatine hydrochloride, baicalin, baicalin and ammonium glycyrrhizinate as the index, HPLC was used to establish the content determination method of 10 components in the extract of Gegen Qinlian decoction, and their comprehensive weighted scores were performed. The effects of extraction method, ethanol concentration, extraction time, extraction times and solvent multiple on the active components of Gegen Qinlian decoction were investigated by single factor test. Combined with Box-Behnken response surface method, the comprehensive weighted score was used as the evaluation index to optimize the optimal extraction process of Gegen Qinlian decoction. RESULTS: The optimal extraction process

关 键 词：葛根芩连汤 含量测定 多指标综合加权评分法 Box-Behnken响应面法 提取工艺 

分 类 号：R28[医药卫生—中药学]