腐殖酸对硫酸盐还原菌厌氧还原硫酸盐的抑制效果及其机理研究  

The Role and Mechanism of Humic Acid in Inhibiting the Anaerobic Production of Sulfate by Sulfate Reducing Bacteria

作　　者：马彬彬 吴敏[1] 王致远 李恭霞 赵刘柱 陈世阳 

机构地区：[1]同济大学,污染控制与资源化研究国家重点实验室,上海 [2]上海市政工程设计研究总院(集团)有限公司,上海 [3]同济大学建筑设计研究院(集团)有限公司,上海

出　　处：《水污染及处理》2020年第1期24-30,共7页Water Pollution and Treatment

摘　　要：在含大量SRB的污泥中投加HA,在厌氧条件下进行摇瓶反应,结果表明:HA对SRB产生H2S和硫化物有强烈的抑制效果,80~200 mg/L的HA在对产生H2S的抑制效果超过80%,对硫化物的产生抑制效果超过50%;HA能够促进SRB对电子供体的利用,投加200 mg/L HA的SRB对电子供体的总利用率为97.88%,相比未投加HA的SRB提高了20.62%;投加AQDS的摇瓶实验间接验证了投加HA扰乱了SRB还原SO42- 的电子传递体系,并成为SRB代谢的最终电子受体;HA对SRB代谢过程中的两种关键酶APS还原酶和SO32- 还原酶都有显著的抑制作用,其抑制效果分别为44.59%和42.53%。The HA was added to sludge containing a large amount of SRB and reacted under anaerobic condi-tions. The results showed that HA strongly inhibited the production of H2S and sulfides by SRB. The effect is that 80 to 200 mg/L of HA has an inhibitory effect on H2S production of more than 80% and an inhibitory effect on sulfide production of more than 50%. HA can promote the use of SRB for electron donors. The total utilization of SRB for 200 mg/L HA for electron donors is 97.88%, which is 20.62% higher than that for SRB without HA. The addition of AQDS shake flask experiments indirectly verified that dosing HA disrupted the SRB-reduced SO42- electron transport system and became the ultimate electron acceptor for SRB metabolism. HA has a significant inhibitory effect on two key enzymes in the metabolism of SRB:APS reductase and sulfite reductase, with inhibitory effects of 44.59% and 42.53%, respectively.

关 键 词：腐殖酸 硫酸盐还原菌 硫化氢 电子传递体系 微生物 酶活性 

分 类 号：X70[环境科学与工程—环境工程]