Detection of aac(3)IIc, aac(6)Ib, armA Genes Coding for Escherichia coli Resistance to Aminoglycosides in Burkina Faso  

作　　者：Pamane Djagbare Christelle Nadembega Tani Sagna Abdoul Karim Ouattara Emmanuel Sampo Théodora Zohoncon Moussa Ouedraogo Marius Belemgnegre Dorcas Obiri-Yeboah Jacques Simpore Pamane Djagbare;Christelle Nadembega;Tani Sagna;Abdoul Karim Ouattara;Emmanuel Sampo;Théodora Zohoncon;Moussa Ouedraogo;Marius Belemgnegre;Dorcas Obiri-Yeboah;Jacques Simpore(Laboratory of Molecular Biology and Genetics, LaBioGene, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso;Institute for Research in Health Sciences/National Center for Scientific and Technological Research, IRSS-CNRST, Ouagadougou, Burkina Faso;Biomedical Laboratory of the Schiphra Hospital, Ouagadougou, Burkina Faso;Biomedical Laboratory of Saint Camille Hospital HOSCO, Ouagadougou, Burkina Faso;School of Medical Sciences, Department of Microbiology and Immunology, University of Cape Coast, University Post Office, Cape Coast, Ghana)

机构地区：[1]Laboratory of Molecular Biology and Genetics, LaBioGene, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso [2]Institute for Research in Health Sciences/National Center for Scientific and Technological Research, IRSS-CNRST, Ouagadougou, Burkina Faso [3]Biomedical Laboratory of the Schiphra Hospital, Ouagadougou, Burkina Faso [4]Biomedical Laboratory of Saint Camille Hospital HOSCO, Ouagadougou, Burkina Faso [5]School of Medical Sciences, Department of Microbiology and Immunology, University of Cape Coast, University Post Office, Cape Coast, Ghana

出　　处：《Advances in Infectious Diseases》2023年第4期574-585,共12页传染病进展（英文）

摘　　要：Background and Prupose: Antibiotic resistance is a major global health concern. In addition to the existing data on the prevalence of bacterial resistance to antibiotics, there are patchy data on bacterial resistance to aminoglycosides in Burkina Faso. In this study, we determined the prevalence of aminoglycoside resistance genes in E. coli, including aac(3)-IIc, aac(6)-Ib and armA in Ouagadougou, and determined which antibiotics in this class are most affected by resistance. Material and Methods: This study was conducted on 216 E. coli strains collected from the biomedical analysis laboratories of Saint Camille and Schiphra hospitals. E. coli strains were isolated from pus and urine samples collected between September 2018 and January 2019. Antibiotic susceptibility testing was performed using aminoglycosides, β-lactams, fluoroquinolones, and sulfonamides. Aminoglycoside resistance genes were detected in strains with at least one aminoglycoside resistance gene using conventional/multiplex PCR. Results: Aminoglycoside resistance was observed in 46.8% (101/216) of strains. The resistance rates were respectively 45.37% for Tobramycin, 32.40% for Gentamicin, 14.81% for Kanamycin, 2.31% for Netilmicin, 1.84% for Neomycin, and 0.46% for Amikacin. PCR showed that 86 strains (85.15%) possessed the aac(3)-IIc gene, 71 strains or 70.30%) possessed the aac(6’)-Ib gene, and nine strains (8.91%) possessed the armA gene. Conclusion: Aminoglycoside resistance in pathogenic E. coli strains is mainly due to the presence of the aac(3’)-IIc and aac(6’)-Ib genes. The presence of armA was first reported in Burkina Faso. Netilmicin, Neomycin and Amikacin are good therapeutic options for treating urinary tract and pus-forming infections.Background and Prupose: Antibiotic resistance is a major global health concern. In addition to the existing data on the prevalence of bacterial resistance to antibiotics, there are patchy data on bacterial resistance to aminoglycosides in Burkina Faso. In this study, we determined the prevalence of aminoglycoside resistance genes in E. coli, including aac(3)-IIc, aac(6)-Ib and armA in Ouagadougou, and determined which antibiotics in this class are most affected by resistance. Material and Methods: This study was conducted on 216 E. coli strains collected from the biomedical analysis laboratories of Saint Camille and Schiphra hospitals. E. coli strains were isolated from pus and urine samples collected between September 2018 and January 2019. Antibiotic susceptibility testing was performed using aminoglycosides, β-lactams, fluoroquinolones, and sulfonamides. Aminoglycoside resistance genes were detected in strains with at least one aminoglycoside resistance gene using conventional/multiplex PCR. Results: Aminoglycoside resistance was observed in 46.8% (101/216) of strains. The resistance rates were respectively 45.37% for Tobramycin, 32.40% for Gentamicin, 14.81% for Kanamycin, 2.31% for Netilmicin, 1.84% for Neomycin, and 0.46% for Amikacin. PCR showed that 86 strains (85.15%) possessed the aac(3)-IIc gene, 71 strains or 70.30%) possessed the aac(6’)-Ib gene, and nine strains (8.91%) possessed the armA gene. Conclusion: Aminoglycoside resistance in pathogenic E. coli strains is mainly due to the presence of the aac(3’)-IIc and aac(6’)-Ib genes. The presence of armA was first reported in Burkina Faso. Netilmicin, Neomycin and Amikacin are good therapeutic options for treating urinary tract and pus-forming infections.

关 键 词：E. coli Aminoglycoside Resistance acc(3’)-IIc aac(6’)-Ib ARMA Burkina Faso 

分 类 号：R37[医药卫生—病原生物学]