Integration of 1H-NMR-Based Metabolomics Approach with Traditional Chinese Pulse Diagnosis to Evaluate the Qi-Enhancing Activity of Herbal Tea  

作　　者：Chia-Hsin Lin Po-Jung Huang Chung-Hsuan Wang Mo-Rong Xu Sheng-Yang Wang Chia-Hsin Lin;Po-Jung Huang;Chung-Hsuan Wang;Mo-Rong Xu;Sheng-Yang Wang(Department of Forestry, Chung Hsing University, Taichung;Fu Kang Traditional Chinese Medicine Clinic, Nantou;Special Crop and Metabolome Discipline Cluster, Academy Circle Economy, Chung Hsing University, Taichung;Agricultural Biotechnology Research Institute, Academia Sinica, Taipei City)

机构地区：[1]Department of Forestry, Chung Hsing University, Taichung [2]Fu Kang Traditional Chinese Medicine Clinic, Nantou [3]Special Crop and Metabolome Discipline Cluster, Academy Circle Economy, Chung Hsing University, Taichung [4]Agricultural Biotechnology Research Institute, Academia Sinica, Taipei City

出　　处：《Chinese Medicine》2024年第3期49-74,共26页中医（英文）

摘　　要：Human metabolism is intricately linked to an individual’s health status. Regardless of living habits, it will be reflected in the metabolic characteristics of urine. The utilization of the 1H NMR-based metabolomics method has enabled examine the metabolomic changes in urine under various physiology conditions, providing valuable insights into metabolites. In this particular study, volunteers were divided into two groups based on the strength of their spleen pulses, using the pulse diagnosis method employed in traditional Chinese medicine. Subsequently, their urine samples were analyzed, revealing notable variances in urea, creatinine, citric acid, succinic acid, trimethylamine-N-oxide (TMAO), alanine, hippuric acid, and glycine between the two groups. Interestingly, individuals with weak spleen pulses showed significant improvements after consuming herbal tea. Furthermore, we conducted LC-MS analysis on herbal tea and performed adenosine triphosphate (ATP) activity tests on the C2C12 mouse skeletal muscle cell line. The results indicated that within a reasonable concentration range, exposure to herbal tea led to an increase in the mitochondrial ATP production capacity of C2C12 cells. These findings shed light on the relationship between traditional Chinese medicine pulse diagnosis and urine metabolites, highlighting their potential as non-invasive and straightforward health assessment indicators. They can aid in the preliminary determination of necessary dietary and lifestyle changes to enhance overall bodily health.Human metabolism is intricately linked to an individual’s health status. Regardless of living habits, it will be reflected in the metabolic characteristics of urine. The utilization of the 1H NMR-based metabolomics method has enabled examine the metabolomic changes in urine under various physiology conditions, providing valuable insights into metabolites. In this particular study, volunteers were divided into two groups based on the strength of their spleen pulses, using the pulse diagnosis method employed in traditional Chinese medicine. Subsequently, their urine samples were analyzed, revealing notable variances in urea, creatinine, citric acid, succinic acid, trimethylamine-N-oxide (TMAO), alanine, hippuric acid, and glycine between the two groups. Interestingly, individuals with weak spleen pulses showed significant improvements after consuming herbal tea. Furthermore, we conducted LC-MS analysis on herbal tea and performed adenosine triphosphate (ATP) activity tests on the C2C12 mouse skeletal muscle cell line. The results indicated that within a reasonable concentration range, exposure to herbal tea led to an increase in the mitochondrial ATP production capacity of C2C12 cells. These findings shed light on the relationship between traditional Chinese medicine pulse diagnosis and urine metabolites, highlighting their potential as non-invasive and straightforward health assessment indicators. They can aid in the preliminary determination of necessary dietary and lifestyle changes to enhance overall bodily health.

关 键 词：1H-NMR Metabolomics Traditional Chinese Pulse Diagnosis Qi-Enhancing Herbal Tea 

分 类 号：R28[医药卫生—中药学]