Study on the Protective Mechanism of Acupuncture Regulating ICAM-5 mRNA Expression in Rats with Ischemic Stroke  

作　　者：Yating Shen Ximei Xie Xiu Bai Hua Bai Xiaofan Chang Yuanyuan Tang Qiuyu Li Yating Shen;Ximei Xie;Xiu Bai;Hua Bai;Xiaofan Chang;Yuanyuan Tang;Qiuyu Li(Xi’an Hospital of Traditional Chinese Medicine, Xi’an, China;Shaanxi University of traditional Chinese Medicine, Xianyang, China)

机构地区：[1]Xi’an Hospital of Traditional Chinese Medicine, Xi’an, China [2]Shaanxi University of traditional Chinese Medicine, Xianyang, China

出　　处：《Journal of Behavioral and Brain Science》2021年第12期313-321,共9页行为与脑科学期刊（英文）

摘　　要：<b>Objective:</b> The objective is to investigate whether acupuncture can protect brain by regulating the expression of ICAM-5 mRNA in brain tissue. <b>Methods:</b> Male SD rats were used to construct a middle cerebral artery occlusion reperfusion injury (Middle cerebral artery occlusion, MCAO) model, and the rats were randomly divided into 4 groups, namely the normal group (group A) and the sham operation group (group B), MCAO group (group C), MCAO + acupuncture group (group D);real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the expression of ICAM-5 mRNA in the brain tissue of the ischemic area, and HE staining and TUNEL were used to detect the level of neuronal apoptosis to evaluate the therapeutic effect. <b>Results:</b> Acupuncture can improve neuronal morphology and reduce neuronal apoptosis in rats with ischemic stroke. RT-qPCR: compared with group A and group B, the expression level of ICAM-5 mRNA in the brain tissues of group C and group D decreased, while compared with group C, The expression of ICAM-5 mRNA in the brain tissue of group D was significantly up-regulated (P < 0.05). There was no statistical difference between group A and group B (P > 0.05). <b>Conclusion:</b> The effectiveness of acupuncture in the treatment of ischemic stroke may achieve neuroprotection by up-regulating the expression level of ICAM-5 mRNA.<b>Objective:</b> The objective is to investigate whether acupuncture can protect brain by regulating the expression of ICAM-5 mRNA in brain tissue. <b>Methods:</b> Male SD rats were used to construct a middle cerebral artery occlusion reperfusion injury (Middle cerebral artery occlusion, MCAO) model, and the rats were randomly divided into 4 groups, namely the normal group (group A) and the sham operation group (group B), MCAO group (group C), MCAO + acupuncture group (group D);real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the expression of ICAM-5 mRNA in the brain tissue of the ischemic area, and HE staining and TUNEL were used to detect the level of neuronal apoptosis to evaluate the therapeutic effect. <b>Results:</b> Acupuncture can improve neuronal morphology and reduce neuronal apoptosis in rats with ischemic stroke. RT-qPCR: compared with group A and group B, the expression level of ICAM-5 mRNA in the brain tissues of group C and group D decreased, while compared with group C, The expression of ICAM-5 mRNA in the brain tissue of group D was significantly up-regulated (P < 0.05). There was no statistical difference between group A and group B (P > 0.05). <b>Conclusion:</b> The effectiveness of acupuncture in the treatment of ischemic stroke may achieve neuroprotection by up-regulating the expression level of ICAM-5 mRNA.

关 键 词：Ischemic Stroke ACUPUNCTURE ICAM-5 mRNA Mechanism Study 

分 类 号：R24[医药卫生—中医临床基础]